Cell Viability of Porous Poly(d,l-lactic acid)/Vertically Aligned Carbon Nanotubes/Nanohydroxyapatite Scaffolds for Osteochondral Tissue Engineering

Author(s)

Stocco, Thiago Domingues ^{[1, 2]} ; Antonioli, Eliane ^[3] ; Vaz Elias, Conceicao de Maria ^[4] ; Manzolli Rodrigues, Bruno Vinicius ^[4] ; Waltrick de Brito Siqueira, Idalia Aparecida ^[5] ; Ferretti, Mario ^[3] ; Marciano, Fernanda Roberta ^[4] ; Lobo, Anderson Oliveira ^[6]

Affiliation

^[1] Univ Estadual Campinas, Fac Med Sci, BR-13083887 Sao Paulo - Brazil

^[2] Univ Santo Amaro, Fac Physiotherapy, BR-04829300 Sao Paulo - Brazil

^[3] Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo - Brazil

^[4] Brasil Univ, Sci & Technol Inst, BR-08230030 Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Inst Sci & Technol, BR-12231280 Sao Paulo - Brazil

^[6] UFPI Fed Univ Piaui, LIMAV Interdisciplinary Lab Adv Mat, BR-64049550 Teresina, Piaui - Brazil

• Document type: Journal article
• Source: MATERIALS; v. 12, n. 6 MAR 2 2019.
• Web of Science Citations: 0

Abstract

Treatment of articular cartilage lesions remains an important challenge. Frequently the bone located below the cartilage is also damaged, resulting in defects known as osteochondral lesions. Tissue engineering has emerged as a potential approach to treat cartilage and osteochondral defects. The principal challenge of osteochondral tissue engineering is to create a scaffold with potential to regenerate both cartilage and the subchondral bone involved, considering the intrinsic properties of each tissue. Recent nanocomposites based on the incorporation of nanoscale fillers into polymer matrix have shown promising results for the treatment of osteochondral defects. In this present study, it was performed using the recently developed methodologies (electrodeposition and immersion in simulated body fluid) to obtain porous superhydrophilic poly(d,l-lactic acid)/vertically aligned carbon nanotubes/nanohydroxyapatite (PDLLA/VACNT-O:nHAp) nanocomposite scaffolds, to analyze cell behavior and gene expression of chondrocytes, and then assess the applicability of this nanobiomaterial for osteochondral regenerative medicine. The results demonstrate that PDLLA/VACNT-O:nHAp nanocomposite supports chondrocytes adhesion and decreases type I Collagen mRNA expression. Therefore, these findings suggest the possibility of novel nanobiomaterial as a scaffold for osteochondral tissue engineering applications. (AU)

• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Research Grants - Young Investigators Grants