Change in the antimicrobial resistance profile of Pseudomonas aeruginosa from soil after exposure to herbicides

Author(s)

Braz, Vania Santos ^[1] ; Silva Moretto, Jessica Aparecida ^[1] ; Tonelli Fernandes, Ana Flavia ^[1] ; Stehling, Eliana Guedes ^[1]

Affiliation

^[1] Univ Sao Paulo USP Ribeirao Preto, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto - Brazil

• Document type: Journal article
• Source: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN; v. 54, n. 4, p. 290-293, APR 3 2019.
• Web of Science Citations: 0

Abstract

The extensive use of pesticides represents a risk to human health and to the environment. This study aimed to investigate if the exposure to atrazine and diuron, two herbicides widely used in Brazil, could induce changes in the susceptibility profile to aztreonam, colistin and polymyxin B antimicrobials in isolates of P. aeruginosa obtained from soil samples by using the determination of minimum inhibitory concentration (MIC) test. Three isolates had an increase of MIC to aztreonam after exposure to both herbicides and one isolate did not show any MIC change. The MexAB-OprM efflux pump has already been upregulated in these isolates and the herbicides atrazine and diuron did not increase MexAB-OprM overexpression. Therefore, the decrease in aztreonam susceptibility was not directly related to this pump, suggesting that probably other mechanisms should be involved. (AU)

• FAPESP's process: 14/02806-5 - Development of bacterial consortia for the biodegradation of atrazine
• Grantee: Ana Flavia Tonelli Scaranello
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/18990-2 - Investigation of quinolone resistance mechanisms in environmental bacterial isolates
• Grantee: Eliana Guedes Stehling
• Support Opportunities: Regular Research Grants