Lack of Association between IL-6 Polymorphisms and Haplotypes with Gastric Cancer

Author(s)

dos Santos, Monica Pezenatto ^[1] ; Sallas, Mayara Luciana ^[1] ; Zapparoli, Diana ^[1] ; Orcini, Wilson Aparecido ^[1] ; Chen, Elizabeth ^[2] ; Cardoso Smith, Marilia de Arruda ^[2] ; Marques Payao, Spencer L. ^[3] ; Rasmussen, Lucas Trevizani ^{[3, 1]}

Affiliation

^[1] USC, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Sao Paulo - Brazil

^[3] Fac Med Marilia FAMEMA, Hemoctr, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Cellular Biochemistry; v. 120, n. 6, p. 9448-9454, JUN 2019.
• Web of Science Citations: 2

Abstract

The process of combating neoplasms and mononuclear cells, and during H. pylori infection, several pro-inflammatory and anti-inflammatory cytokines are synthesized. In view of the involvement of the IL-6 law and the presence of H. pylori in the development of gastric diseases, the present study aimed to characterize the promoter-region polymorphism -597 (G/A) (rs1800797), -572 (C/G) (rs1800796), and -174 (G/C) (rs1800795) by PCR-RFLP in 375 gastric biopsy specimens from patients with peptic symptoms. A total of 375 samples were analyzed: 87 patients (without lesion without gastric tissue); 236 patients with gastritis and 52 patients with gastric cancer analyzed the PCR-RFLP techniques. All the results were normalized in relation to the presence of H. pylori. The frequencies of the three polymorphisms were compared in the Control vs Gastritis groups and a statistically significant test observed: -174 (G/C) (OR: 1.27; 95% CI: 0.84-1.93; P=0.26), 572 (C/G) (OR: 1.42; 95% CI: 0.78-2.59; P=0.25), and 597 (G/A) (OR: 0.98; 95% CI, 0.64-1.52; P=0.94). Similar results were obtained when the gastric cancer group was compared to the control group: -174 (G/C) (OR: 1.27; 95% CI: 0.66-2.47; P=0.47), -572 (C/G) (OR: 1.07; 95% CI: 0.43-2.68; P=0.88), and -597 (G/A) (OR: 1.01; 95% CI, 0.5-0.9; P=0.99). The haplotypes were and were not observed statistically significant differences. In conclusion, we found no correlations between any of the three polymorphisms in the IL-6 gene analyzed in this study and a higher risk of gastritis or gastric cancer. (AU)

• FAPESP's process: 18/08481-1 - Gene and Protein Interaction among Components of the Inflammatory, Apoptosis and microRNAs Pathways in Gastric Disease and Gastric Cancer
• Grantee: Lucas Trevizani Rasmussen
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/11371-5 - Analysis of Expression of Micro RNAs and Genes Associated with Apoptosis and Chronic Inflammation in Gastric Diseases: A Possible Relation with Helicobacter pylori
• Grantee: Lucas Trevizani Rasmussen
• Support Opportunities: Regular Research Grants