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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Novel process of hydration, followed by incubation and thermal processing, for high isoflavone bioconversion in soybeans

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Author(s):
Salces, Franz R. [1] ; Rostagno, Mauricio A. [2] ; Amaya-Farfan, Jaime [1]
Total Authors: 3
Affiliation:
[1] Univ Campinas UNICAMP, Sch Food Engn FEA, Food & Nutr Dept DEPAN, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Sch Appl Sci FCA, R Pedro Zaccaria 1300, POB 1068, BR-13484350 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Food Research International; v. 121, p. 691-696, JUL 2019.
Web of Science Citations: 0
Abstract

The potentially bioavailable aglyconic isoflavone content of soybeans was increased by a process based on the controlled hydration of whole beans, followed by an incubation step and cooking. For developing the process, the effects of three operation variables: temperature, intermittent soaking and incubation time on the isoflavone profile of the processed soybeans were assessed. By hydrating the whole beans under controlled conditions (54 degrees C; 15 rpm for a rotating soaking basket) and holding the beans for an appropriate incubation time, it was possible to substantially increase the total aglycone content from (mu mol.10(-2).g(-1)) similar to 5 in the raw, to similar to 95 in the processed soybean. A conventional thermal treatment (1 kg.cm(-2), 5 min), necessary to attain the nutritional and sensory characteristics, produced additional hydrolysis of glucosides, accounting for extra 14% of total aglycone yield. The entire process avoided the need to grind the bean and permitted an overall 21.8-fold increase (per mole basis) conversion of all forms of isoflavone glucosides to aglycones, particularly to the (S)-equol precursor, daidzein, and with minimal back-diffusion or leaching to the outside medium. (AU)

FAPESP's process: 13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology
Grantee:Maurício Ariel Rostagno
Support Opportunities: Research Grants - Young Investigators Grants