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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improving direct immunoassay response by layer-by-layer films of gold nanoparticles - Antibody conjugate towards label-free detection

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Author(s):
Camilo, Douglas E. [1] ; Miyazaki, Celina M. [1] ; Shimizu, Flavio M. [2] ; Ferreira, Marystela [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Rod Joao Leme dos Santos, Km 110, BR-18052780 Sorocaba, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 102, p. 315-323, SEP 2019.
Web of Science Citations: 1
Abstract

The conjugation of nanoparticles with antibodies has been successfully applied in sandwich immunoassays for detecting cancer biomarkers. However, two antibodies are necessary to perform such experiment, being one of them functionalized with a signal label for optical or electrochemical assay. This approach is time and cost consuming compared to direct label-free immunoassays. In this study, we propose the synthesis of gold nano particles conjugated to anti-PSA antibody to produce a label-free impedimetric inununosensors based on nanostructured Layer-by-Layer (LbL) films. Prostate-specific antigen (PSA) detection was performed by electro-chemical impedance spectroscopy demonstrating a detection mechanism governed by Langmuir-Freundlich adsorption model. This strategy provided a significant sensitivity using 10-fold less antibody than conventional inununosensors, i.e. decreasing costs using a simple approach, with a limit of detection of 0.17 ng mL(-1) and an analytical range of 0.1-20 ng mL(-1) indicating that our sensor is potentially useful for clinical applications. (AU)

FAPESP's process: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing
Grantee:Celina Massumi Miyazaki
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
Grantee:Roberto Mendonça Faria
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/19387-0 - LbL (LAYER-BY-LAYER) and Langmuir-Blodgett (LB) nanostructured films with emodin for systems carriers.
Grantee:Marystela Ferreira
Support Opportunities: Regular Research Grants