Prospective study of a Bothrops jararacussu venom batch (Bj2015) - phospholipase A(2) activity, immunogenicity, neurotoxicity, and myotoxicity parameters

Author(s):Show less -

Fontana Oliveira, Isadora Caruso ^[1] ; Dalla Mora, Adriana ^[1] ; Neto, Jairo Braga ^[2] ; de Oliveira, Akila Lara ^[1] ; Harder, Carolina ^[3] ; Hanai-Yoshida, Valquiria Miwa ^[2] ; Cogo, Jose Carlos ^{[4, 5]} ; Floriano, Rafael Stuani ^[6] ; Bonventi Junior, Waldemar ^[2] ; Oliveira, Sara de Jesus ^[1] ; Oshima-Franco, Yoko ^[1]

Affiliation

^[1] Univ Sorocaba, Postgrad Program Pharmaceut Sci, Pharm & Vet Med Grad Courses, Sorocaba - Brazil

^[2] Univ Sorocaba, Postgrad Program Technol & Environm Proc & Comp E, Sorocaba - Brazil

^[3] Univ Paulista, Sao Paulo - Brazil

^[4] Univ Brazil, Postgrad Program Biomed Engn, Sao Paulo - Brazil

^[5] Univ Brazil, Postgrad Program Bioengn, Sao Paulo - Brazil

^[6] Univ Estadual Campinas, Dept Pharmacol, Campinas, SP - Brazil

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. 33, n. 16, p. 2417-2421, AUG 18 2019.
• Web of Science Citations: 0

Abstract

Bothrops jararacussu venom's (Bj2015) batch was biomonitored quarterly for one year to assess phospholipase A(2) (PLA(2)) activity, immunogenicity, neurotoxicity, and myotoxicity. In silico models were applied to evaluate losses using decay model and recoveries by predictive trend analysis. Mice were immunized with Bj2015. Antibodies were detected by double-immunodiffusion and total protein and albumin were measured. Neuromuscular blockade-induced by 40 mu g mL(-1) venom solution was carried out using mouse nerve phrenic-diaphragm preparation. Resulting muscles were submitted to light microscopy to evaluate the myotoxicity. PLA(2) activity of 0.1 mg mL(-1) Bj2015 was measured using 4-nitro-3-(octanoyloxy)benzoic acid as substrate. Over time, greater losses occurred in neurotoxicity than PLA(2), but not in myotoxicity and immunogenicity. Concluding, the neurotoxicity decrease can be related to enzymatic losses, including PLA(2). Depending on the purpose of use, the collected venom responds on a long time, avoiding unnecessary new collections, improving life quality of animals in captivity and increasing their longevity. {[}GRAPHICS] . (AU)

• FAPESP's process: 15/01420-9 - Development and efficacy evaluation (in vitro and in vivo) of a hemoperfuser cartridge for the antiophidian treatment
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/08271-0 - IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants