Brazil nut intake increases circulating miR-454-3p and miR-584-5p in obese women

Author(s)

Reis, Bruna Zavarize ^{[1, 2]} ; Silva Duarte, Graziela Biude ^{[1, 2]} ; Vargas-Mendez, Ernesto ^{[1, 2]} ; Pinto Ferreira, Ludmila Rodrigues ^{[3, 4]} ; Barbosa Jr, Fernando ; Cercato, Cintia ^{[5, 6]} ; Rogero, Marcelo Macedo ^{[7, 8, 9]} ; Franciscato Cozzolino, Silvia Maria ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[2] Av Prof Lineu Prestes 580, Bloco 14, BR-05508000 Sao Paulo, SP - Brazil

^[3] Fed Univ Minas Gerais UFMG, Inst Biol Sci ICB, Dept Morphol, RSBL, Belo Horizonte, MG - Brazil

^[4] Av Antonio Carlos 6627, Bloco J, 4 Andar, Sala 158, BR-31270910 Belo Horizonte, MG - Brazil

^[5] Univ Sao Paulo, Clin Hosp, Sch Med, Div Endocrinol & Metab, Sao Paulo - Brazil

^[6] Rua Dr Eneas de Carvalho Aguiar 155, BR-05403000 Sao Paulo, SP - Brazil

^[7] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, Sao Paulo - Brazil

^[8] Sao Paulo Res Fdn, CEPID FAPESP, Food Res Ctr FoRC, Res Innovat & Disseminat Ctr, BR-05468140 Sao Paulo - Brazil

^[9] Av Dr Arnaldo 715, BR-01246904 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Nutrition Research; v. 67, p. 40-52, JUL 2019.
• Web of Science Citations: 0

Abstract

The Brazil nut is an excellent source of selenium (Se), an essential micronutrient for human health. In this study, we hypothesized that Brazil nut intake modulates circulating microRNAs (miRNAs) in obese women and aimed to evaluate the effects of this nut intake on circulating miRNAs in women with obesity or metabolic syndrome (MetS). A randomized controlled clinical trial was conducted on 54 subjects recruited from the Clinical Hospital in Sao Paulo, Brazil. Patients were randomly assigned to 2 groups: a Brazil nut group (BN group, n = 29) and a control group (CO group, n = 25); both were monitored for 2 months. BN group members were instructed to consume 1 Brazil nut (approximately 1261 g/Se) per day; CO group members were instructed not to consume any. Biochemical parameters related to Se status and 25 circulating miRNAs in plasma were evaluated in all patients both at baseline and after 2 months. Expression levels of 2 miRNAs (miR-454-3p and miR-584-5p) were significantly increased after Brazil nut intake. To investigate the effect of MetS on circulating miRNAs at baseline, we performed comparisons between women with MetS (n = 23) and women without MetS (others, n = 31). Circulating miR-375 levels were significantly lower (P =.012) in women with MetS. In conclusion, our findings suggested that a daily intake of 1 Brazil nut increased circulating miR-454-3p and miR-584-5p expression levels in obese women, and our network analysis indicated a link between Se intake, vitamin D metabolism, and calcium homeostasis. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 15/02906-2 - Effect of supplementation with brazil nut (Bertholettia excelsa H.B.K) on inflammation, oxidative stress and the expression of selenoprotein p and microRNAs in obese women
• Grantee: Silvia Maria Franciscato Cozzolino
• Support Opportunities: Regular Research Grants