Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil

Author(s):Show less -

Leal, Elcio ^[1] ; Luchs, Adriana ^[2] ; de Padua Milagres, Flavio Augusto ^{[3, 4, 5, 6]} ; Komninakis, Shirley Vasconcelos ^{[7, 8]} ; Gill, Danielle Elise ^[9] ; Brito Sayao Lobato, Marcia Cristina Alves ^{[4, 6]} ; Brustulin, Rafael ^{[4, 5, 6]} ; das Chagas, Rogerio Togisaki ^{[4, 6]} ; Neves dos Santos Abrao, Maria de Fatima ^{[4, 6]} ; de Deus Alves Soares, Cassia Vitoria ^{[4, 6]} ; Villanova, Fabiola ^[1] ; Witkin, Steven S. ^{[9, 10]} ; Deng, Xutao ^{[11, 12]} ; Sabino, Ester Cerdeira ^{[3, 9]} ; Delwart, Eric ^{[11, 12]} ; da Costa, Antonio Charlys ^[9]

Affiliation:Show less -

^[1] Univ Fed Para, Inst Ciencias Biol, BR-66075000 Belem, Para - Brazil

^[2] Adolfo Lutz Inst, Ctr Virol, Lab Doencas Enter, BR-01246000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, LIM-46, BR-01246903 Sao Paulo - Brazil

^[4] Secretaria Saude Tocantins, BR-77453000 Tocantins - Brazil

^[5] Univ Fed Tocantins, Inst Ciencias Biol, BR-77001090 Tocantins - Brazil

^[6] Lab Cent Saude Publ Tocantins LACEN TO, BR-77016330 Tocantins - Brazil

^[7] Fac Med ABC, BR-09060870 Santo Andre - Brazil

^[8] Univ Fed Sao Paulo, Lab Retrovirol, BR-04023062 Sao Paulo - Brazil

^[9] Univ Sao Paulo, Inst Med Trop, BR-05403000 Sao Paulo - Brazil

^[10] Weill Cornell Med, Dept Obstet & Gynecol, 1300 York Ave, New York, NY 10065 - USA

^[11] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 - USA

^[12] Blood Syst Res Inst, San Francisco, CA 94143 - USA

• Document type: Journal article
• Source: Viruses-Basel; v. 11, n. 6 JUN 2019.
• Web of Science Citations: 0

Abstract

We characterized the 24 nearly full-length genomes of human parechoviruses (PeV) from children in the north of Brazil. The initial phylogenetic analysis indicated that 17 strains belonged to genotype 1, 5 to genotype 4, and 1 to genotype 17. A more detailed analysis revealed a high frequency of recombinant strains (58%): A total of 14 of our PeV-As were chimeric, with four distinct recombination patterns identified. Five strains were composed of genotypes 1 and 5 (Rec1/5); five strains shared a complex mosaic pattern formed by genotypes 4, 5, and 17 (Rec4/17/5); two strains were composed of genotypes 1 and 17 (Rec1/17); and two strains were composed of genotype 1 and an undetermined strain (Rec1/und). Coalescent analysis based on the Vp1 gene, which is free of recombination, indicated that the recombinant strains most likely arose in this region approximately 30 years ago. They are present in high frequencies and are circulating in different small and isolated cities in the state of Tocantins. Further studies will be needed to establish whether the detected recombinant strains have been replacing parental strains or if they are co-circulating in distinct frequencies in Tocantins. (AU)

• FAPESP's process: 15/12944-9 - Investigating the evoltution of animal rotavirus strains infecting humans
• Grantee: Adriana Luchs
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Regular Research Grants