Acute physical exercise increases APPL1/PI3K signaling in the hypothalamus of lean mice

Author(s):Show less -

Gaspar, Rafael Calais ^[1] ; Munoz, Vitor Rosetto ^[1] ; Kuga, Gabriel Keine ^[2] ; Branco Ramos Nakandakari, Susana Castelo ^[3] ; Crisol, Barbara Moreira ^[1] ; Lenhare, Lucien ^[1] ; Breda, Leonardo ^[1] ; Botezelli, Jose Diego ^[1] ; Sant'Ana, Marcella Ramos ^[3] ; da Silva, Adelino S. R. ^{[4, 5]} ; Cintra, Dennys Esper ^{[6, 3]} ; de Moura, Leandro Pereira ^{[6, 7, 1]} ; Ropelle, Eduardo Rochete ^{[6, 7, 1]} ; Pauli, Jose Rodrigo ^{[6, 7, 1]}

Affiliation

^[1] Univ Campinas UNICAMP, Lab Mol Biol Exercise LaBMEx, 1300 Pedro Zaccaria St Postal Code 1068, BR-13484350 Limeira - Brazil

^[2] Sao Paulo State Univ UNESP, Postgrad Program Movement Sci, Rio Claro - Brazil

^[3] Univ Campinas UNICAMP, Lab Nutr Genom LabGeN, Limeira - Brazil

^[4] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Postgrad Program Phys Educ & Sport, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto - Brazil

^[6] Univ Estadual Campinas, OCRC, Lab Cell Signaling, Campinas, SP - Brazil

^[7] Univ Campinas UNICAMP, CEPECE Ctr Res Sport Sci, Sch Appl Sci, Limeira - Brazil

• Document type: Journal article
• Source: European Journal of Neuroscience; v. 50, n. 7 JULY 2019.
• Web of Science Citations: 0

Abstract

Adiponectin is an adipokine that acts in the control of energy homeostasis. The adaptor protein containing the pleckstrin homology domain, phosphotyrosine-binding domain, and leucine zipper motif 1 (APPL1) is a key protein in the adiponectin signaling. The APPL1 mediates a positive effect on the insulin signaling through the interaction with the phosphoinositide 3-kinase (PI3K). Thus, the present study aimed to explore the effects of an acute physical exercise session on the hypothalamic adiponectin signaling. Firstly, using bioinformatics analysis, we found a negative correlation between hypothalamic APPL1 mRNA levels and food consumption in several strains of genetically diverse BXD mice. Also, the mice and the human database revealed a positive correlation between the levels of APPL1 mRNA and PI3K mRNA. At the molecular level, the exercised mice showed increased APPL1 and PI3K (p110) protein contents in the hypothalamus of Swiss mice. Furthermore, the exercise increases co-localization between APPL1 and PI3K p110 predominantly in neurons of the arcuate nucleus of hypothalamus (ARC). Finally, we found an acute exercise session reduced the food intake 5 hr after the end of fasting. In conclusion, our results indicate that physical exercise reduces the food intake and increases some proteins related to adiponectin pathway in the hypothalamus of lean mice. (AU)

• FAPESP's process: 16/18488-8 - The role of physical exercise on molecular pathways of glucose uptake in GLUT-4 knockout mice
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants