Human papillomavirus is not associated to non-small cell lung cancer: data from a prospective cross-sectional study

Author(s)

Silva, Estela Maria ^[1] ; Mariano, Vania Sammartino ^[1] ; Aguiar Pastrez, Paula Roberta ^[1] ; Pinto, Miguel Cordoba ^[2] ; Nunes, Emily Montosa ^[3] ; Sichero, Laura ^[3] ; Villa, Luisa Lina ^{[4, 3]} ; Scapulatempo-Neto, Cristovam ^{[1, 5]} ; Syrjanen, Kari Juhani ^{[6, 5]} ; Longatto-Filho, Adhemar ^{[1, 7, 8, 9, 10]}

Affiliation

^[1] Barretos Canc Hosp, Pio XII Fdn, Teaching & Res Inst, Mol Oncol Res Ctr, Barretos - Brazil

^[2] Barretos Canc Hosp, Dept Chest, Pio XII Fdn, Barretos - Brazil

^[3] ICESP, Ctr Translat Res Oncol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Sao Paulo - Brazil

^[5] COI Inst, Rio De Janeiro - Brazil

^[6] Dept Clin Res Biohit Oyj, Helsinki - Finland

^[7] Univ Sao Paulo, Med Lab Med Invest LIM 14, Fac Med, Dept Pathol, Sao Paulo - Brazil

^[8] Univ Minho, Res Inst Life & Hlth Sci ICVS, Sch Med, Braga - Portugal

^[9] 3Bs Associated Lab Govt Portugal, ICVS, Braga - Portugal

^[10] Univ Minho, Surg Sci Res Domain Life & Hlth Sci Res Inst ICVS, Sch Med, Campus Gualtar, P-4710057 Braga - Portugal

• Document type: Journal article
• Source: INFECTIOUS AGENTS AND CANCER; v. 14, n. 1 AUG 2 2019.
• Web of Science Citations: 0

Abstract

Background The pathogenesis of lung cancer is triggered by a combination of genetic and environmental factors, being the tobacco smoke the most important risk factor. Nevertheless, the incidence of lung cancer in non-smokers is gradually increasing, which demands the search for different other etiological factors such as occupational exposure, previous lung disease, diet among others. In the early 80's a theory linked specific types of human papillomavirus (HPV) to lung cancer due to morphological similarities of a subset of bronchial squamous cell carcinomas with other HPV-induced cancers. Since then, several studies revealed variable rates of HPV DNA detection. The current study aimed to provide accurate information on the prevalence of HPV DNA in lung cancer. Methods Biopsies were collected from 77 newly diagnosed non-small cell lung cancer (NSCLC) patients treated at the Thoracic Oncology Department at Barretos Cancer Hospital. The samples were formalin fixed and paraffin embedded (FFPE), histologic analysis was performed by an experienced pathologist. DNA was extracted from FFPE material using a commercial extraction kit and HPV DNA detection was evaluated by multiplex PCR and HPV16 specific real-time PCR. Results HPV was not identified in any of the samples analysed (69). Conclusions Our data demonstrated a lack of HPV DNA in a series of NSCL cancers. (AU)

• FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
• Grantee: Luisa Lina Villa
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 14/23414-8 - Characterization of the immunological profile of patients with non-small cell lung cancer and its potential association with human papillomavirus
• Grantee: Estela Maria Silva
• Support type: Scholarships in Brazil - Master