L-Arginine supplementation blunts resistance exercise improvement in rats with chronic kidney disease

Author(s):Show less -

Souza, Michel Kendy ^[1] ; Moraes, Milton Rocha ^{[2, 1]} ; Rosa, Thiago Santos ^{[2, 3]} ; Passos, Clevia Santos ^[1] ; Passos Neves, Rodrigo Vanerson ^{[2, 3]} ; Haro, Anderson Sola ^[1] ; Cenedeze, Marcos Antonio ^[1] ; Alarcon Arias, Simone Costa ^[4] ; Fujihara, Clarice Kazue ^[4] ; Teixeira, Simone Aparecida ^[5] ; Muscara, Marcelo Nicolas ^[5] ; Saraiva Camara, Niels Olsen ^{[6, 1]} ; Pacheco e Silva Filho, Alvaro ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Dept Med, Div Nephrol, Sao Paulo - Brazil

^[2] Catholic Univ Brasilia UCB, Grad Program Phys Educ, Brasilia, DF - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Dept Med, Postgrad Program Translat Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo FMUSP, Fac Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 232, SEP 1 2019.
• Web of Science Citations: 0

Abstract

Chronic kidney disease (CKD) patients present L-arginine (L-arg) deficiency and L-arg supplementation has been used as a treatment. In addition, sarcopenia is another common problem in CKD population, resistance training (RT) is one of the conservative strategies developed to prevent CKD progression, and however there are no evidences of a combination of these two strategies to treat CKD outcomes. The aim of this study was to evaluate the effects of oral L-arg supplementation combined with RT in an experimental model of CKD. Twenty-five Munich-Wistar male rats, 8-week-old were divided in 5 groups: Sham (sedentary control), Nx (CKD sedentary), Nx L-arg (CKD sedentary supplemented with 2% of L-arg), Nx RT (CKD exercised) Nx RT + L-arg (CKD exercised and supplemented with 2% of L-arg). CKD model was obtained by a subtotal 5/6 nephrectomy. RT was performed on a ladder climbing, three weekly sessions on non-consecutive days, with an intensity of 70% maximum carrying capacity. They were submitted to RT and/or L-arg supplementation for 10 weeks. There was a significant improvement in muscle strength, renal function, anti-inflammatory cytokines, arginase metabolism and renal fibrosis after RT. However, the combination of RT and L-arg impaired all the improvements promoted by RT alone. The L-arg supplementation alone did not impair renal fibrosis and renal function. In conclusion, RT improved inflammatory balance, muscle strength, renal function and consequently decreased renal fibrosis. Nevertheless, the association with L-arg supplementation prevented all these effects promoted by RT. (AU)

• FAPESP's process: 17/05264-7 - Cell metabolism, microbiota and immune system: new paradigms in renal diseases physiopathology
• Grantee: Niels Olsen Saraiva Câmara
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/02270-2 - New cellular, molecular and immunological mechanisms involved in acute and chronic renal injury: the search for new therapeutical approaches
• Grantee: Niels Olsen Saraiva Câmara
• Support Opportunities: Research Projects - Thematic Grants