Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi

Full text
Author(s):
Luísa Czamanski Nora [1] ; Relber Aguiar Gonçales [2] ; Leonardo Martins-Santana [3] ; Beatriz Henriques Ferreira [4] ; Fernando Rodrigues [5] ; Rafael Silva-Rocha [6]
Total Authors: 6
Affiliation:
[1] Universidade de São Paulo. Departmento de Biologia Celular e Molecular Biology e Bioagentes Patogênicos. Systems and Synthetic Biology Laboratory - Brasil
[2] Universidade de São Paulo. Departmento de Biologia Celular e Molecular Biology e Bioagentes Patogênicos. Immunochemistry and Glycobiology Laboratory - Brasil
[3] Universidade de São Paulo. Departmento de Biologia Celular e Molecular Biology e Bioagentes Patogênicos. Systems and Synthetic Biology Laboratory - Brasil
[4] University of Minho. Life and Health Sciences Research Institute (ICVS). School of Health Sciences - Portugal
[5] University of Minho. Life and Health Sciences Research Institute (ICVS). School of Health Sciences - Portugal
[6] Universidade de São Paulo. Departmento de Biologia Celular e Molecular Biology e Bioagentes Patogênicos. Systems and Synthetic Biology Laboratory - Brasil
Total Affiliations: 6
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 2, p. 395-398, 2019-06-13.
Abstract

Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation: minimalist, synthetic, modular, and available in four different versions, and these can still be readily modified using a few primers and few cloning steps. (AU)

FAPESP's process: 16/03763-3 - Unveiling the mechanisms of gene regulation at the single cell level in Trichoderma reesei
Grantee:Luísa Czamanski Nora
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters
Grantee:Rafael Silva Rocha
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/01946-3 - Construction of synthetic promoters for the expression of cellulases using novel cis-regulatory elements in Trichoderma reesei
Grantee:Leonardo Martins Santana
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/22561-7 - Overexpression of paracoccin: phenotype and virulence of Paracoccidioides brasiliensis strains
Grantee:Relber Aguiar Gonçales
Support Opportunities: Scholarships in Brazil - Doctorate