Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil

Author(s):Show less -

Audrey Cilli ^[1] ; Adriana Luchs ^[2] ; Elcio Leal ^[3] ; Danielle Gill ; Flavio Augusto de Pádua Milagres ; Shirley Vasconcelos Komninakis ; Rafael Brustulin ; Maria da Aparecida Rodrigues Teles ; Márcia Cristina Alves Brito Sayão Lobato ; Rogério Togisaki das Chagas ; Maria de Fátima Neves dos Santos Abrão ; Cassia Vitória de Deus Alves Soares ; Xutao Deng ; Eric Delwart ; Ester Cerdeira Sabino ; Antonio Charlys da Costa

Affiliation:Show less -

^[1] Instituto Adolfo Lutz. Centro de Virologia. Núcleo de Doenças Entéricas - Brasil

^[2] Instituto Adolfo Lutz. Centro de Virologia. Núcleo de Doenças Entéricas - Brasil

^[3] Universidade Federal do Pará. Instituto de Ciências Biológicas - Brasil

• Document type: Journal article
• Source: Memórias do Instituto Oswaldo Cruz; v. 114, 2019-04-04.

Abstract

Human sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characterization in the country precedes that of the present study. This is the first complete genome sequence of genotype GI.3 in Brazil. The data obtained in this investigation can contribute to the augmentation of the database on the molecular diversity of HSaVs strains circulating in Brazil, and to the improvement of current typing protocols. (AU)

• FAPESP's process: 15/12944-9 - Investigating the evoltution of animal rotavirus strains infecting humans
• Grantee: Adriana Luchs
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral