Development and application of new nanoestructurated chemical sensors based on bio...
| Full text | |
| Author(s): |
Bergamin, Bruna
[1, 2]
;
Pupin, Rafael R.
[1, 2]
;
Wong, Ademar
[1, 2]
;
Sotomayor, Maria D. P. T.
[1, 2]
Total Authors: 4
|
| Affiliation: | [1] Univ Estadual Paulista UNESP, Inst Quim, Dept Quim Analit, BR-14801970 Araraquara, SP - Brazil
[2] Inst Nacl Tecnol Alternat Deteccao Avaliacao Toxi, BR-14801970 Araraquara, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Journal of the Brazilian Chemical Society; v. 30, n. 11, SI, p. 2344-2354, NOV 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
A polyurethane composite electrode modified with magnetic nanoparticles (mag; Fe3O4) coated with molecularly imprinted polymers (MIPs) was developed for the electrochemical determination of estradiol valerate. Chemical and morphological analyses of the mag-MIP were performed using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), with the aim of characterizing this new material. The efficiency of adsorption of estradiol valerate by the mag-MIP was tested in binding experiments using an electrochemical method. For this, different electrodes (mag-MIP / graphite-epoxy composite (GEC), mag-non-imprinted polymer (NIP) / GEC, mag / GEC, and GEC) were evaluated by square-wave voltammetry (SWV). After 10 min at open circuit potential, increases in the current signal of 1.9, 2.1, and 3.0 times were obtained, comparing the electrochemical response of the mag-MIP / GEC sensor to the mag-NIP / GEC, mag / GEC, and GEC sensors, respectively. Under optimized conditions, the mag-MIP / GEC showed a linear concentration range for estradiol valerate of 5.0 x 10(-7) to 7.5 x 10(-4) mol L-1 and a limit of detection of 1.0 x 10(-8) mol L-1. The proposed sensor was applied in the analysis of pharmaceutical, human urine, and river water samples. The recovery values determined using high performance liquid chromatography (HPLC)-UV and the electrochemical method were in agreement and were near 100%, demonstrating the reliability of the proposed method. (AU) | |
| FAPESP's process: | 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies |
| Grantee: | Maria Valnice Boldrin |
| Support type: | Research Projects - Thematic Grants |