A New Electrochemical Platform Based on a Polyurethane Composite Electrode Modified with Magnetic Nanoparticles Coated with Molecularly Imprinted Polymer for the Determination of Estradiol Valerate in Different Matrices

Author(s)

Bergamin, Bruna ^{[1, 2]} ; Pupin, Rafael R. ^{[1, 2]} ; Wong, Ademar ^{[1, 2]} ; Sotomayor, Maria D. P. T. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Paulista UNESP, Inst Quim, Dept Quim Analit, BR-14801970 Araraquara, SP - Brazil

^[2] Inst Nacl Tecnol Alternat Deteccao Avaliacao Toxi, BR-14801970 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 30, n. 11, SI, p. 2344-2354, NOV 2019.
• Web of Science Citations: 0

Abstract

A polyurethane composite electrode modified with magnetic nanoparticles (mag; Fe3O4) coated with molecularly imprinted polymers (MIPs) was developed for the electrochemical determination of estradiol valerate. Chemical and morphological analyses of the mag-MIP were performed using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), with the aim of characterizing this new material. The efficiency of adsorption of estradiol valerate by the mag-MIP was tested in binding experiments using an electrochemical method. For this, different electrodes (mag-MIP / graphite-epoxy composite (GEC), mag-non-imprinted polymer (NIP) / GEC, mag / GEC, and GEC) were evaluated by square-wave voltammetry (SWV). After 10 min at open circuit potential, increases in the current signal of 1.9, 2.1, and 3.0 times were obtained, comparing the electrochemical response of the mag-MIP / GEC sensor to the mag-NIP / GEC, mag / GEC, and GEC sensors, respectively. Under optimized conditions, the mag-MIP / GEC showed a linear concentration range for estradiol valerate of 5.0 x 10(-7) to 7.5 x 10(-4) mol L-1 and a limit of detection of 1.0 x 10(-8) mol L-1. The proposed sensor was applied in the analysis of pharmaceutical, human urine, and river water samples. The recovery values determined using high performance liquid chromatography (HPLC)-UV and the electrochemical method were in agreement and were near 100%, demonstrating the reliability of the proposed method. (AU)

• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants