| Full text | |
| Author(s): |
do Amaral, Cristiane Candida
[1]
;
Fernandes, Geisa Ferreira
[2]
;
Rodrigues, Anderson Messias
[2]
;
Burger, Eva
[3]
;
de Camargo, Zoilo Pires
[2]
Total Authors: 5
|
| Affiliation: | [1] Fed Univ Sao Paulo UNIFESP, Discipline Infect Dis, Dept Med, Sao Paulo - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Discipline Cellular Biol, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[3] Fed Univ Alfenas UNIFAL, Dept Microbiol & Immunol, Alfenas - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | PLoS One; v. 14, n. 7 JUL 2 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Background Paracoccidioidomycosis (PCM) is a systemic mycosis commonly found in Latin America that is caused by distinct species of Paracoccidioides genus: Paracoccidioides brasiliensis complex (S1, PS2, PS3 and PS4) and Paracoccidioides lutzii. Its pathobiology has been recently explored by different approaches to clarify the mechanisms of host-pathogen interactions underpinning PCM. The diversity of clinical forms of this disease has been attributed to both host-and fungus-related factors. Methodology/Principal findings For better understanding of the molecular underpinnings of host-fungus interactions, we evaluated in vivo virulence of nine Paracoccidioides brasiliensis complex isolates and correlated it to protein expression profiles obtained by two-dimensional gel electrophoresis. Based on the recovery of viable fungi from mouse organs, the isolates were classified as those having low, moderate, or high virulence. Highly virulent isolates overexpressed proteins related to adhesion process and stress response, probably indicating important roles of those fungal proteins in regulating the colonization capacity, survival, and ability to escape host immune system reaction. Moreover, highly virulent isolates exhibited enhanced expression of glycolytic pathway enzymes concomitantly with repressed expression of succinylCoA ligase beta chain, a protein related to the tricarboxylic acid cycle. Conclusions/Significance Our findings may point to the mechanisms used by highly virulent P. brasiliensis isolates to withstand host immune reactions and to adapt to transient iron availability as strategies to survive and overcome stress conditions inside the host. (AU) | |
| FAPESP's process: | 09/54024-2 - Molecular biology and proteomics of medically interesting fungi: Paracoccidioides brasiliensis and Sporothrix schenckii |
| Grantee: | Zoilo Pires de Camargo |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/27265-5 - Molecular epidemiology and genomic perspectives on the evolution and spread of emerging fungal pathogens |
| Grantee: | Anderson Messias Rodrigues |
| Support Opportunities: | Research Grants - Young Investigators Grants |