Growth hormone/STAT5 signaling in proopiomelanocortin neurons regulates glucoprivic hyperphagia

Author(s)

Quaresma, Paula G. F. ^[1] ; Teixeira, Pryscila D. S. ^[1] ; Furigo, Isadora C. ^[1] ; Wasinski, Frederick ^[1] ; Couto, Gisele C. ^[1] ; Frazao, Renata ^[2] ; List, Edward O. ^{[3, 4]} ; Kopchick, John J. ^{[3, 4]} ; Donato Jr, Jose

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo, SP - Brazil

^[3] Ohio Univ, Edison Biotechnol Inst, Athens, OH 45701 - USA

^[4] Ohio Univ, Heritage Coll Osteopath Med, Athens, OH 45701 - USA

• Document type: Journal article
• Source: Molecular and Cellular Endocrinology; v. 498, DEC 1 2019.
• Web of Science Citations: 2

Abstract

Several hypothalamic neuronal populations are directly responsive to growth hormone (GH) and central GH action regulates glucose and energy homeostasis. However, the potential role of GH signaling in proopiomelanocortin (POMC) neurons has not been studied yet. Thus, we investigated whether POMC neurons are responsive to GH and if ablation of GH receptor (GHR) or STAT5 in POMC cells leads to metabolic imbalances. Approximately 60% of POMC neurons of the arcuate nucleus exhibited STAT5 phosphorylation after intracerebroventricular GH injection. Ablation of GHR or STAT5 in POMC cells did not affect energy or glucose homeostasis. However, glucoprivic hyperphagia was blunted in male and female GHR knockout mice, and in male POMC-specific STAT5 knockout mice. Additionally, the absence of GHR in POMC neurons decreased glycemia during prolonged food restriction in male mice. Thus, GH action in POMC neurons regulates glucoprivic hyperphagia as well as blood glucose levels during prolonged food restriction. (AU)

• FAPESP's process: 17/25281-3 - Effects of growth hormone on POMC, cholinergic and hypothalamic paraventricular nucleus neurons: implications for energy and glycemic metabolism control
• Grantee: Paula Gabriele Fernandes Quaresma Bergonsi
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/20897-3 - Role of orexin neurons as mediators of the central effects induced by growth hormone
• Grantee: Frederick Wasinski
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 17/21840-8 - Growth hormone signaling in the brain as a mediator of puberty and fertility
• Grantee: Renata Frazão
• Support type: Regular Research Grants
• FAPESP's process: 17/04006-4 - The importance of growth hormone's action on NPY/AgRP neurons
• Grantee: Gisele Cristina Lopes Couto Spiri
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 17/02983-2 - The role of growth hormone in the brain: relevance for neural functions and in disease
• Grantee: Jose Donato Junior
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 16/09679-4 - Central effects of growth hormone on energetic metabolism and glicemic control
• Grantee: Isadora Clivatti Furigo
• Support type: Scholarships in Brazil - Post-Doctorate