| Full text | |
| Author(s): |
Nora, Luisa Czamanski
[1]
;
Westmann, Caua Antunes
[1]
;
Guazzaroni, Maria-Eugenia
[2]
;
Siddaiah, Chandranayaka
[3]
;
Gupta, Vijai Kumar
[4]
;
Silva-Rocha, Rafael
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Mysore, Dept Biotechnol, Mysuru - India
[4] Tallinn Univ Technol, Sch Sci, Dept Chem & Biotechnol, ERA Chair Green Chem, EE-12618 Tallinn - Estonia
Total Affiliations: 4
|
| Document type: | Review article |
| Source: | BIOTECHNOLOGY ADVANCES; v. 37, n. 8 DEC 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
A key challenge for domesticating alternative cultivable microorganisms with biotechnological potential lies in the development of innovative technologies. Within this framework, a myriad of genetic tools has flourished, allowing the design and manipulation of complex synthetic circuits and genomes to become the general rule in many laboratories rather than the exception. More recently, with the development of novel technologies such as DNA automated synthesis/sequencing and powerful computational tools, molecular biology has entered the synthetic biology era. In the beginning, most of these technologies were established in traditional microbial models (known as chassis in the synthetic biology framework) such as Escherichia coli and Saccharomyces cerevisiae, enabling fast advances in the field and the validation of fundamental proofs of concept. However, it soon became clear that these organisms, although extremely useful for prototyping many genetic tools, were not ideal for a wide range of biotechnological tasks due to intrinsic limitations in their molecular/physiological properties. Over the last decade, researchers have been facing the great challenge of shifting from these model systems to non-conventional chassis with endogenous capacities for dealing with specific tasks. The key to address these issues includes the generation of narrow and broad host plasmid-based molecular tools and the development of novel methods for engineering genomes through homologous recombination systems, CRISPR/Cas9 and other alternative methods. Here, we address the most recent advances in plasmid-based tools for the construction of novel cell factories, including a guide for helping with ``build-your-own{''} microbial host. (AU) | |
| FAPESP's process: | 16/03763-3 - Unveiling the mechanisms of gene regulation at the single cell level in Trichoderma reesei |
| Grantee: | Luísa Czamanski Nora |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries |
| Grantee: | María Eugenia Guazzaroni |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/05472-6 - Synthetic biology approaches to engineer/mine novel regulatory elements in bacteria |
| Grantee: | Cauã Antunes Westmann |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters |
| Grantee: | Rafael Silva Rocha |
| Support Opportunities: | Research Grants - Young Investigators Grants |