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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Regulation and neurochemical identity of melanin-concentrating hormone neurones in the preoptic area of lactating mice

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Author(s):
Teixeira, Pryscila D. S. [1] ; Wasinski, Frederick [1] ; Lima, Leandro B. [1] ; Frazao, Renata [2] ; Bittencourt, Jackson C. [2] ; Donato Jr, Jose
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Av Prof Lineu Prestes 1524, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Neuroendocrinology; v. 32, n. 2 DEC 2019.
Web of Science Citations: 0
Abstract

Neurones expressing the melanin-concentrating hormone (MCH) can be found in the medial preoptic area (mPOA) and ventral aspects of the periventricular preoptic nucleus of rats by mid-to-late lactation and this expression disappears after weaning. The transitory expression of MCH in the preoptic area suggests a role for these neurones in the control of the end of lactation. However, the neurochemical identity of mPOA MCH neurones and the regulatory factors that control the transient MCH expression remain largely unknown, especially in the mouse. In the present study, we showed that mice also present the transitory expression of MCH in the mPOA at late lactation. mPOA MCH cells did not colocalise significantly with markers of GABAergic (VGAT), glutamatergic (VGLUT2 and VGLUT3) or dopaminergic (tyrosine hydroxylase) neurones. mPOA MCH cells also did not express Kiss1 or oxytocin. By contrast, approximately 70% and 90% of mPOA MCH neurones colocalised with oestrogen receptor alpha and prolactin-induced phosphorylated signal transducer and activator of transcription 5 (STAT5), respectively. Finally, we demonstrated that the number of MCH neurones in the mPOA is significantly higher in females during the first lactation, compared to mice on the second lactation or pregnant mice during the first lactation or brain-specific STAT5 knockout mice during the first lactation. In summary, our findings indicate that MCH neurones in the mPOA of lactating mice are sensitive to oestrogens and prolactin. Thus, mPOA MCH expression is possibly influenced by hormonal variations. Furthermore, the STAT5 signalling pathway is likely involved in the regulation of MCH expression in the mPOA of lactating mice. (AU)

FAPESP's process: 16/20897-3 - Role of orexin neurons as mediators of the central effects induced by growth hormone
Grantee:Frederick Wasinski
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/21840-8 - Growth hormone signaling in the brain as a mediator of puberty and fertility
Grantee:Renata Frazão
Support Opportunities: Regular Research Grants
FAPESP's process: 17/02983-2 - The role of growth hormone in the brain: relevance for neural functions and in disease
Grantee:Jose Donato Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/02224-1 - The existence of neurogenesis during the lactation period
Grantee:Jackson Cioni Bittencourt
Support Opportunities: Research Projects - Thematic Grants