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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation

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Author(s):
Pereira, Priscilla A. T. [1, 2] ; Bitencourt, Claudia S. [1, 3] ; Reis, Mouzarllem B. [1] ; Frantz, Fabiani G. [1] ; Sorgi, Carlos A. [1] ; Souza, Camila O. S. [1] ; Silva, Celio L. [4] ; Gardinassi, Luiz G. [1] ; Faccioli, Lucia H. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Analises Clin Toxicol & Bromatol, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Fundacao Dracenense Educ & Cultura, Fac Dracena, Dept Med, Rua Bahia 332, BR-17900000 Dracena, SP - Brazil
[3] Ctr Univ Fac Associadas Ao Ensino, Largo Engn Paulo de A Samdeville 15, Sao Joao Da Boa Vista, SP - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Inflammation Research; v. 69, n. 1 NOV 2019.
Web of Science Citations: 0
Abstract

Objective and design Investigate survival outcomes, and immunological and metabolomic effects of hyaluronidase (Hz) treatment during mouse models of acute inflammation and sepsis. Methods Survival of C57Bl/6 mice was monitored after lethal challenge with lipopolysaccharide (LPS) or cecal and ligation puncture (CLP)-induced sepsis and treated with Hz or saline. Mice were also challenged with LPS and treated with Hz for leukocyte counting, cytokine quantification and determination of metabolomic profiles in the peritoneal fluid. Results Hz treatment improved survival outcomes after lethal challenge with LPS or CLP-induced sepsis. LPS challenge promoted acute neutrophil accumulation and production of interleukin-1 beta (IL-1 beta) and IL-6 in the peritoneum, whereas Hz treatment suppressed neutrophil infiltration and cytokine production. We further characterized the metabolomic alterations caused by LPS challenge, which predicted activity of metabolic pathways related to fatty acids and eicosanoids. Hz treatment had a profound effect over the metabolic response, reflected by reductions of the relative levels of fatty acids. Conclusion Collectively, these data demonstrate that Hz treatment is associated with metabolic reprogramming of pathways that sustain the inflammatory response. (AU)

FAPESP's process: 07/04741-4 - Study of activity of soluble hyaluronidases or encapsulated in association or not with MK886
Grantee:Claudia da Silva Bitencourt
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/07125-6 - New functional aspects of eicosanoids
Grantee:Lúcia Helena Faccioli
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/07169-5 - Lipid mediators as regulators of immune response
Grantee:Lúcia Helena Faccioli
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/00658-1 - New functional aspects of eicosanoids
Grantee:Lúcia Helena Faccioli
Support type: Multi-user Equipment Program