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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of effectiveness of 45S5 bioglass doped with niobium for repairing critical-sized bone defect in in vitro and in vivo models

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Author(s):
de Souza, Ucas P. L. [1] ; Lopes, Joao H. [2] ; Ferreira, V, Filipe ; Martin, Richard A. [3, 4] ; Bertran, Celso A. [5] ; Camilli, Jose A. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, UNICAMP, BR-13083862 Campinas, SP - Brazil
[2] Aeronaut Inst Technol ITA, Dept Chem, Div Fundamental Sci IEF, BR-12228900 Sao Jose Dos Campos, SP - Brazil
[3] Aston Univ, Sch Engn, Birmingham, W Midlands - England
[4] Aston Univ, Aston Inst Mat Res, Birmingham, W Midlands - England
[5] Univ Estadual Campinas, Inst Chem, Dept Phys Chem, UNICAMP, Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 108, n. 3 NOV 2019.
Web of Science Citations: 0
Abstract

Here, we investigated the biocompatibility of a bioactive sodium calcium silicate glass containing 2.6 mol% Nb2O5 (denoted BGPN2.6) and compare the results with the archetypal 45S5 bioglass. The glass bioactivity was tested using a range of in vitro and in vivo experiments to assess its suitability for bone regeneration applications. in vitro studies consisted of assessing the cytocompatibility of the BGPN2.6 glass with bone-marrow-derived mesenchymal stem cells (BM-MSCs). Systemic biocompatibility was verified by means of the quantification of biochemical markers and histopathology of liver, kidneys, and muscles. The glass genotoxicity was assessed using the micronucleus test. The regeneration of a calvarial defect was assessed using both qualitative and quantitative analysis of three-dimensional microcomputed tomography images. The BGPN2.6 glass was not cytotoxic to BM-MSCs. It is systemically biocompatible causing no signs of damage to high metabolic and excretory organs such as the liver and kidneys. No mutagenic potential was observed in the micronucleus test. MicroCT images showed that BGPN2.6 was able to nearly fully regenerate a critical-sized calvarial defect and was far superior to standard 45S5 Bioglass. Defects filled with BGPN2.6 glass showed over 90% coverage compare to just 66% for 45S5 Bioglass. For one animal the defect was completely filled in 8 weeks. These results clearly show that Nb-containing bioactive glasses are a safe and effective biomaterial for bone replacement. (AU)

FAPESP's process: 10/05394-9 - Bioglasses derived from 45S5: effects of Nb2O5 or surface modification with Ca2+ on the glass structure and bioactivity
Grantee:João Henrique Lopes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/24372-2 - TISSUE RESPONSE IN A CRITICAL SIZED DEFECT OF RATS CALVARIA TREATED WITH A NIOBIUM DOPED BIOACTIVE GLASS: IN VITRO AND IN VIVO STUDY.
Grantee:Jose Angelo Camilli
Support Opportunities: Regular Research Grants