Comparative data on effects of alkaline pretreatments and enzymatic hydrolysis on bioemulsifier production from sugarcane straw by Cutaneotrichosporon mucoides

Author(s)

Jimenez, Itzcoatl M. ^[1] ; Chandel, Anuj K. ^[1] ; Marcelino, Paulo R. F. ^[1] ; Anjos, Virgilio ^[2] ; Costa, Cassiano Batesttin ^[2] ; Bell, Maria Jose V. ^[2] ; Pereira, Barbara ^[1] ; da Silva, Silvio S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Biotechnol, Engn Sch Lorena, Estr Municipal Campinho S-N, BR-12602810 Sao Paulo - Brazil

^[2] Univ Fed Juiz de Fora, Dept Phys, Engn & Mat Spect Grp, BR-36036900 Juiz De Fora, MG - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 301, APR 2020.
• Web of Science Citations: 0

Abstract

Bioemulsifiers are surface active compounds which could be potentially used in food processing, cosmetic sector and oil recovery. Sugarcane straw (SS), was used as the raw substrate for the production of bio-emulsifiers (BE) by Cutaneotrichosporon mucoides. Three different delignification strategies using dilute sodium hydroxide, sodium sulfite and ammonium hydroxide followed by enzymatic hydrolysis (Cellic CTec 2, 7.5% total solids, 15 FPU/g, 72 h) were studied. Enzyme hydrolysis of ammonium hydroxide pretreated SS showed a maximum of 62.19 +/- 0.74 g/l total reducing sugars with 88.35% hydrolytic efficiency (HE) followed by sodium hydroxide (60.06 +/- 0.33 g/l; 85.40% HE) and sodium sulfite pretreated SS (57.22 +/- 0.52 g/l; 84.71% HE), respectively. The ultrastructure of SS (native and delignified) by fourier transform-infrared and near infrared spectroscopy, revealed notable structural differences. The fermentation of hydrolysates by C. mucoides into bioemulsifiers showing emulsification index (EI) of 54.33%, 48.66% and 32.66% from sodium sulfite, sodium hydroxide, and ammonium hydroxide pretreated SS, respectively. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 08/57926-4 - Research and development aiming at the integrated exploitation of sugarcane bagasse for the biotechnological production of lignocellulosic ethanol
• Grantee: Silvio Silvério da Silva
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 16/14852-7 - A sustainable solution against neglected tropical diseases using biosurfactants produced by yeasts in sugarcane bagasse hemicellulosic hydrolyzate
• Grantee: Paulo Ricardo Franco Marcelino
• Support Opportunities: Scholarships in Brazil - Post-Doctoral