Identification and genomic features of halotolerant extended-spectrum-beta-lactamase (CTX-M)-producing Escherichia coli in urban-impacted coastal waters, Southeast Brazil

Author(s)

Fernandes, Miriam R. ^[1] ; Sellera, Fabio P. ^[2] ; Moura, Quezia ^[3] ; Esposito, Fernanda ^[1] ; Sabino, Caetano P. ^[1] ; Lincopan, Nilton ^{[4, 1]}

Affiliation

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil

^[3] Fed Univ Grande Dourados, Fac Hlth Sci, Dourados, MS - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Marine Pollution Bulletin; v. 150, JAN 2020.
• Web of Science Citations: 0

Abstract

We report the occurrence and genomic analysis of extended-spectrum beta-lactamase (CTX-M)-producing Escherichia coli in anthropogenically polluted coastal waters of Southeast Brazil. E. coli strains belonging to sequence types (STs) ST10, ST38, ST155 and ST1284 exhibited a wide resistome, with genes conferring resistance to medically relevant antimicrobials and heavy metals, and a halophilic behavior (tolerance to 9-10% NaCl). These findings suggest a heavy contamination in this area by critical priority bacteria adapted to marine environments, which might have negative impacts on human and ocean health. (AU)

• FAPESP's process: 15/13527-2 - Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods
• Grantee: Miriam Rodriguez Fernandes
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support type: Regular Research Grants
• FAPESP's process: 16/03044-7 - Genetic diversity, virulence genes and antimicrobial resistance of Salmonella spp. isolated from poultry slaughterhouse
• Grantee: Daniel Farias Marinho Do Monte
• Support type: Scholarships in Brazil - Doctorate