Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study

Author(s)

Oliveira Prado Bergamo, Mariel Tavares ^[1] ; Ribeiro Vitor, Luciana Lourenco ^[1] ; Lopes, Nathalia Martins ^[2] ; Lourenco Neto, Natalino ^[1] ; Dionisio, Thiago Jose ^[2] ; Oliveira, Rodrigo Cardoso ^[2] ; Rodini, Camila Oliveira ^[2] ; Santos, Carlos Ferreira ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Oliveira, Thais Marchini ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 35, n. 9 FEB 2020.
• Web of Science Citations: 0

Abstract

This study evaluated the viability, proliferation, and protein expression after photobiomodulation (PBM) of stem cell from human exfoliated deciduous teeth (SHED). The groups were the following: G1 (2.5 J/cm2), G2 (3.7 J/cm2), and control (not irradiated). According to the groups, cells were irradiated with InGaAlP diode laser at 660 nm wavelength, continuous mode, and single time application. After 6 h, 12 h, and 24 h from irradiation, the cell viability and proliferation, and the protein expression were analyzed by MTT, crystal violet, and ELISA multiplex assay, respectively. Twenty-four hours after PBM, SHED showed better proliferation. Over time in the supernatant, all groups had an increase at the levels of VEGF-C, VEGF-A, and PLGF. In the lysate, the control and G2 exhibited a decrease of the VEGF-A, PECAM-1, and PLGF expression, while control and G3 decreased VEGF-C, VEGF-A, and PDGF expression. The dosimetries of 2.5 J/cm2 and 3.7 J/cm2 maintained viability, improved proliferation, and synthesis of the angiogenic proteins in the supernatant in the studied periods on SHED. (AU)

• FAPESP's process: 17/11396-3 - Protein gene expression by stem cells from pulp of deciduous teeth after the use of low intensity laser
• Grantee: Mariel Tavares de Oliveira Prado Bergamo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/20316-6 - Gene expression and protein synthesis analysis of dental pulp cells from deciduous teeth after the use of Low Intensity Laser.
• Grantee: Thais Marchini de Oliveira Valarelli
• Support Opportunities: Regular Research Grants