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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Optimization of key factors affecting hydrogen production from coffee waste using factorial design and metagenomic analysis of the microbial community

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Villa Montoya, Alejandra Carolina [1] ; da Silva Mazareli, Raissa Cristina [1] ; Delforno, Tiago Palladino [2] ; Centurion, Victor Borin [2] ; de Oliveira, Valeria Maia [2] ; Silva, Edson Luiz [3] ; Amancio Varesche, Maria Bernadete [1]
Total Authors: 7
[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Lab Biol Proc, Campus 2, BR-13563120 Sao Carlos, SP - Brazil
[2] Campinas Univ, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Campinas, SP - Brazil
[3] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 45, n. 7, p. 4205-4222, FEB 7 2020.
Web of Science Citations: 0

The objective of this study was to evaluate the fermentation conditions that led to the optimization of H-2 production from coffee waste (wastewater, pulp and husk) and the taxonomic and functional characterization of autochthonous microorganisms. Assays in batch reactors with microbial consortium bioaugmentation (bacteria and fungi) evaluated the pH (4.82-8.18), pulp and husk concentration (6.95-17.05 g/L) and headspace factor (33.18-66.82%) by means of rotational central composite design and response surface. Operating conditions in the reactor optimized for 3.04 LH2/Ld were at pH 7.0, 7 g/L pulp and husk and 30% headspace. The main metabolites observed were butyric acid (3838 mg/L), isobutyric acid (506 mg/L), methanol (226 mg/L) and butanol (156 mg/L). Clostridium sp. (87.9%), Lactobacillus sp. (1.7%), Kazachstania sp. (18.6%) and Saccharomyces sp. (16.3%) were the main genera identified in the optimized reactor, which had functional gene diversity for H-2 production, alcoholic fermentation, cellulose degradation, lignin, hemicellulose and phenol. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/20047-0 - Assessment of physicochemical and phylogenetic features in the hydrogen production and Homoacetogenesis from wastes of coffee processing
Grantee:Alejandra Carolina Villa Montoya
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
Grantee:Marcelo Zaiat
Support type: Research Projects - Thematic Grants