| Full text | |
| Author(s): |
Dutra de Oliveira, Rosa Virginia
[1]
;
Sampaio Bonafe, Fernanda Salloume
[1]
;
Palomari Spolidorio, Denise Madalena
[1]
;
Koga-Ito, Cristiane Yumi
[2]
;
de Farias, Aline Leite
[1]
;
Kirker, Kelly R.
[3]
;
James, Garth A.
[3]
;
Brighenti, Fernanda Lourencao
[1]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Sch Dent, BR-14801385 Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sao Jose Campos Inst Sci & Technol, BR-12245000 Sao Jose Dos Campos, SP - Brazil
[3] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | MICROORGANISMS; v. 8, n. 2 FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The study of bacterial interaction between Streptococcus mutans and Actinomyces naeslundii may disclose important features of biofilm interspecies relationships. The aim of this study was to characterize with an emphasis on biofilm formation and composition and metabolic activity-single- and dual-species biofilms of S. mutans or A. naeslundii, and to use a drip flow reactor (DFR) to evaluate biofilm stress responses to 0.2% chlorhexidine diacetate (CHX). Single- and dual-species biofilms were grown for 24 h. The following factors were evaluated: cell viability, biomass and total proteins in the extracellular matrix, 2,3-bis(2-methoxy-4-nitro-5-sulfopheny1)-2H-tetrazolium-5-carboxanilide- {''}XTT{''}-reduction and lactic acid production. To evaluate stress response, biofilms were grown in DFR. Biofilms were treated with CHX or 0.9% sodium chloride (NaCl; control). Biofilms were plated for viability assessment. Confocal laser-scanning microscopy (CLSM) was also performed. Data analysis was carried out at 5% significance level. S. mutans viability and lactic acid production in dual-species biofilms were significantly reduced. S. mutans showed a higher resistance to CHX in dual-species biofilms. Total protein content, biomass and XTT reduction showed no significant differences between single- and dual-species biofilms. CLSM images showed the formation of large clusters in dual-species biofilms. In conclusion, dual-species biofilms reduced S. mutans viability and lactic acid production and increased S. mutans' resistance to chlorhexidine. (AU) | |
| FAPESP's process: | 12/17236-4 - Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro |
| Grantee: | Rosa Virginia Dutra de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/02397-8 - Interactions between cariogenic bacteria: susceptibility to chlorhexidine and Confocal Scanning Laser Microscopy using drip-flow biofilm reactor |
| Grantee: | Rosa Virginia Dutra de Oliveira |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/12326-8 - Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro |
| Grantee: | Fernanda Lourenção Brighenti |
| Support Opportunities: | Regular Research Grants |