| Full text | |
| Author(s): |
Spagolla Napoleao Tavares, Renata
[1]
;
Stuchi Maria-Engler, Silvya
[2]
;
Colepicolo, Pio
[3]
;
Debonsi, Hosana Maria
[1]
;
Schaefer-Korting, Monika
[4]
;
Marx, Uwe
[5]
;
Rigo Gaspar, Lorena
[1]
;
Zoschke, Christian
[4]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Clin & Toxicol Anal Dept, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[4] Free Univ Berlin, Inst Pharm Pharmacol & Toxicol, Konigin Luise Str 2 4, D-14195 Berlin - Germany
[5] TissUse GmbH, Oudenarder Str 16, D-13347 Berlin - Germany
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PHARMACEUTICS; v. 12, n. 2 FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
UV light catalyzes the ozone formation from air pollutants, like nitrogen oxides. Since ozone reacts with cutaneous sebum lipids to peroxides and, thus, promotes inflammation, tumorigenesis, and aging, even broad-spectrum sunscreens cannot properly protect skin. Meanwhile, xanthophylls, like fucoxanthin, proved their antioxidant and cytoprotective functions, but the safety of their topical application in human cell-based models remains unknown. Aiming for a more detailed insight into the cutaneous fucoxanthin toxicity, we assessed the tissue viability according to OECD test guideline no. 439 as well as changes in inflammation (IL-1 alpha, IL-6, IL-8), homeostasis (EGFR, HSPB1) and metabolism (NAT1). First, we proved the suitability of our 24-well-based reconstructed human skin for irritation testing. Next, we dissolved 0.5% fucoxanthin either in alkyl benzoate or in ethanol and applied both solutions onto the tissue surface. None of the solutions decreased RHS viability below 50%. In contrast, fucoxanthin ameliorated the detrimental effects of ethanol and reduced the gene expression of pro-inflammatory interleukins 6 and 8, while increasing NAT1 gene expression. In conclusion, we developed an organ-on-a-chip compatible RHS, being suitable for skin irritation testing beyond tissue viability assessment. Fucoxanthin proved to be non-irritant in RHS and already showed first skin protective effects following topical application. (AU) | |
| FAPESP's process: | 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach |
| Grantee: | Maria Vitória Lopes Badra Bentley |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/06931-4 - Biodiversity of marine algae and metabolites of economical impact |
| Grantee: | Pio Colepicolo Neto |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |