Potential antibacterial and anti-halitosis activity of medicinal plants against oral bacteria

Author(s)

Veloso, Dejanildo J. ^[1] ; Abrao, Fariza ^[2] ; Martins, Carlos H. G. ^{[2, 3]} ; Bronzato, Juliana D. ^[4] ; Gomes, Brenda P. F. A. ^[4] ; Higino, Jane S. ^[1] ; Sampaio, Fabio C. ^[1]

Affiliation

^[1] Univ Fed Paraiba, Hlth Sci Ctr, Campus 1 Lot, Cidade Univ, BR-58051900 Joao Pessoa, Paraiba - Brazil

^[2] Franca Univ UNIFRAN, Appl Microbiol Res Lab, Av Dr Armando de Sales Oliveira 201, Pq Univ, BR-14404600 Franca, SP - Brazil

^[3] Univ Fed Uberlandia, Inst Biomed Sci, Dept Microbiol, Av Para, 1720 Bloco 4C, Sala 4C 202, BR-38400902 Uberlandia, MG - Brazil

^[4] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 110, FEB 2020.
• Web of Science Citations: 0

Abstract

This study aimed to evaluate the in vitro activity of the crude extracts obtained from Caesalpinia ferrea Mart (Juca), Cinnamomum cassia B. (Cinnamon), Mallow sylvestris L. (Mallow), Punica granatum L. (Pomegranate), Rosmarinus officinalis L. (Rosemary), Aeolanthus suaveolens (Als.) Spreng. (Macassa), Sysygium aromaticum L. (Clove), and Tamarindus indica L. (Tamarind) against oral microorganisms (e.g., Fusobacterium nucleatum, Potphyromonas gingivalis, Prevotella intermedia, and Parvimonas micra) that produce volatile sulfur compounds (VSC). The pure extracts were placed in culture medium for the diffusion test in agar. The Minimum Inhibitory Concentration (MIC) was determined by the microdilution method, and microbial growth was assayed with resazurin. Total polyphenols in the extracts were measured by using the Prussian Blue Method. For the salivary sediment test, the sediments were exposed to the Juca and Pomegranate extracts, which was followed by incubation and organoleptic measurements with a monitor (Halimeer (R)) at 1-, 2-, 4-, and 24 -h intervals. The diffusion test revealed mixed results for the extracts. When the zone of inhibition was present, it ranged from 1.6-10.3 mm. The Pomegranate extract was the only extract that inhibited all the evaluated microorganisms; the MIC values ranged from 50 to 400 mu g/mL. The Pomegranate and Juca extracts presented higher levels of polyphenols, 7.3 % and 3.9 %, respectively, and less VSC formation as compared to the negative control. In conclusion, the extracts display antimicrobial activity against the tested microorganisms. The investigated plants have the potential to reduce the main substances related to halitosis of oral origin. (AU)

• FAPESP's process: 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Research Projects - Thematic Grants