Gut Microbiota Profile of Obese Diabetic Women Submitted to Roux-en-Y Gastric Bypass and Its Association with Food Intake and Postoperative Diabetes Remission

Author(s):Show less -

Al Assal, Karina ^[1] ; Prifti, Edi ^{[2, 3]} ; Belda, Eugeni ^[2] ; Sala, Priscila ^[1] ; Clement, Karine ^{[4, 5]} ; Dao, Maria-Carlota ^{[2, 5]} ; Dore, Joel ^[6] ; Levenez, Florence ^[6] ; Taddei, Carla R. ^[7] ; Fonseca, Danielle Cristina ^[1] ; Rocha, Ilanna Marques ^[1] ; Balmant, Bianca Depieri ^[1] ; Thomas, Andrew Maltez ^[8] ; Santo, Marco A. ^[1] ; Dias-Neto, Emmanuel ^{[8, 9]} ; Setubal, Joao Carlos ^{[10, 11]} ; Zucker, Jean-Daniel ^[3] ; Belarmino, Giliane ^[1] ; Torrinhas, Raquel Susana ^[1] ; Waitzberg, Dan L. ^[1]

Affiliation:Show less -

^[1] Univ Sao Paulo, Digest Surg Discipline LIM 35, Sch Med, Dept Gastroenterol, FMUSP, BR-01246903 Sao Paulo - Brazil

^[2] Hop La Pitie Salpetriere, Integr Team, ICAN, F-75013 Paris - France

^[3] Sorbonne Univ, IRD, Unite Modelisat Math & Informat Syst Complexes UM, F-93143 Bondy - France

^[4] Hop La Pitie Salpetriere, AP HP, Dept Nutr, F-75013 Paris - France

^[5] Sorbonne Univ, INSERM, Nutri Res Unit, F-75013 Paris - France

^[6] Univ Paris Saclay, INRA, MetaGenoPolis, AgroParisTech, MICALIS, F-78350 Jouy En Josas - France

^[7] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Anal & Toxicol, BR-05508000 Sao Paulo - Brazil

^[8] CIPE AC Camargo Canc Ctr, Med Genom Lab, BR-01525001 Sao Paulo - Brazil

^[9] Univ Sao Paulo, Fac Med, Inst Psychiat, Lab Neurosci Alzira Denise Hertzog Silva LIM27, BR-05403010 Sao Paulo - Brazil

^[10] Virginia Tech, Biocomplex Inst, Blacksburg, VA 24061 - USA

^[11] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508220 Sao Paulo - Brazil

• Document type: Journal article
• Source: NUTRIENTS; v. 12, n. 2 FEB 2020.
• Web of Science Citations: 2

Abstract

Gut microbiota composition is influenced by environmental factors and has been shown to impact body metabolism. Objective: To assess the gut microbiota profile before and after Roux-en-Y gastric bypass (RYGB) and the correlation with food intake and postoperative type 2 diabetes remission (T2Dr). Design: Gut microbiota profile from obese diabetic women was evaluated before (n = 25) and 3 (n = 20) and 12 months (n = 14) after RYGB, using MiSeq Illumina-based V4 bacterial 16S rRNA gene profiling. Data on food intake (7-day record) and T2Dr (American Diabetes Association (ADA) criteria) were recorded. Results: Preoperatively, the abundance of five bacteria genera differed between patients with (57%) and without T2Dr (p < 0.050). Preoperative gut bacteria genus signature was able to predict the T2Dr status with 0.94 accuracy ROC curve (receiver operating characteristic curve). Postoperatively (vs. preoperative), the relative abundance of some gut bacteria genera changed, the gut microbial richness increased, and the Firmicutes to Bacteroidetes ratio (rFB) decreased (p < 0.05) regardless of T2Dr. Richness levels was correlated with dietary profile pre and postoperatively, mainly displaying positive and inverse correlations with fiber and lipid intakes, respectively (p < 0.05). Conclusions: Gut microbiota profile was influenced by RYGB and correlated with diet and T2Dr preoperatively, suggesting the possibility to assess its composition to predict postoperative T2Dr. (AU)

• FAPESP's process: 15/01507-7 - Microbial community profiling of human cancers
• Grantee: Andrew Maltez Thomas
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/09612-3 - Changes in gene expression in gastric and intestinal tissue of type 2 Diabetes patients submitted to Roux-en-Y gastric bypass
• Grantee: Dan Linetzky Waitzberg
• Support Opportunities: Research Projects - Thematic Grants