Evaluation of the Dot-ELISA as a diagnostic test for human strongyloidiasis based on the detection of IgA in saliva

Author(s):Show less -

Bosqui, Larissa Rodrigues ^[1] ; Corral, Marcelo Andreetta ^[2] ; Levy, Debora ^[2] ; Bydlowski, Sergio Paulo ^[2] ; Borges Gryschek, Ronaldo Cesar ^[2] ; Custodio, Luiz Antonio ^[1] ; Pavanelli, Wander Rogerio ^[1] ; Conchon-Costa, Ivete ^[1] ; Costa-Cruz, Julia Maria ^[3] ; de Paula, Fabiana Martins ^[2] ; Costa, Idessania Nazareth ^[1]

Affiliation

^[1] Univ Estadual Londrina, UEL, Rodovia Celso Garcia Cid Campus Univ, BR-86051990 Londrina, Parana - Brazil

^[2] FMUSP, Hosp Clin, Av Dr Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[3] Univ Fed Uberlandia, UFU, Av Amazonas S-N, Bloco 4C, BR-38400902 Uberlandia, MG - Brazil

• Document type: Journal article
• Source: Acta Tropica; v. 203, MAR 2020.
• Web of Science Citations: 0

Abstract

This study aimed to evaluate the use of saliva samples in the Dot-ELISA test for immunodiagnosis of human strongyloidiasis. The Dot-ELISA presented similar results to the ELISA test, with 70% and 60% sensitivity and 85% and 90% specificity, respectively, for IgA in the saliva. The Dot-ELISA with alternative saliva samples may be a suitable tool for diagnosing human strongyloidiasis, especially in populations with high levels of exposure to helminth. (AU)

• FAPESP's process: 13/03304-0 - Molecular diagnosis by detection of specific DNA in blood samples from rats experimentally infected with Strongyloides venezuelensis
• Grantee: Fabiana Martins de Paula
• Support Opportunities: Regular Research Grants