Tannin supplementation modulates the composition and function of ruminal microbiome in lambs infected with gastrointestinal nematodes

Author(s):Show less -

Correa, Patricia Spoto ^{[1, 2]} ; Mendes, Lucas William ^[1] ; Lemos, Leandro Nascimento ^[1] ; Crouzoulon, Pierre ^[2] ; Niderkorn, Vincent ^[3] ; Hoste, Herve ^[4] ; Costa-Junior, Livio Martins ^[5] ; Tsai, Siu Mui ^[1] ; Faciola, Antonio P. ^[6] ; Abdalla, Adibe Luiz ^[2] ; Louvandini, Helder ^{[6, 2]}

Affiliation

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Lab Mol Cell Biol, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Ctr Nucl Energy Agr, Lab Anim Nutr, BR-13400970 Piracicaba, SP - Brazil

^[3] Univ Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, F-63122 St Genes Champanelle - France

^[4] INRAE, UMR, Chemin Capelles, BP 87614, F-31076 Toulouse - France

^[5] Univ Fed Maranhao, Biol & Hlth Ctr, Ave Portugueses 1966, BR-65080805 Sao Luis, Maranhao - Brazil

^[6] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 - USA

• Document type: Journal article
• Source: FEMS MICROBIOLOGY ECOLOGY; v. 96, n. 3 MAR 2020.
• Web of Science Citations: 5

Abstract

This study was carried out to evaluate the effects of tannin supplementation on ruminal microbiota of sixteen lambs infected and non-infected with Haemonchus contortus and Trichostrongylus colubriformis. Animals were fed with hay, concentrate and supplemented with Acacia mearnsii (A. mearnsii). The animals were divided into four treatments: two control groups without infection, either receiving A. mearnsii (C+) or not (C-), and two infected groups, one with A. mearnsii (I+) and another without A. mearnsii (I-). Ruminal short-chain fatty acids (SCFA) and metagenome sequencing of ruminal microbiota were used to evaluate the effect of tannin and infection on ruminal microbiome. For SCFA, differences were observed only with A. mearnsii. Total SCFA and acetate molar percentage were decreased in C+ and I+ (P<0.05). Butyrate, valerate and isovalerate were higher in lambs that received A. mearnsii in the diet (P<0.05). The infection changed the microbiome structure and decreased the abundance of butyrate-producing microorganisms. In addition, A. mearnsii supplementation also affected the structure the microbial community, increasing the diversity and abundance of the butyrate-producing and probiotics bacteria, amino acid metabolic pathways, purine, pyrimidine and sphingolipid metabolism. Together, our findings indicate that A. mearnsii supplementation modulates important groups related to nitrogen, amino acid, purine and pyrimidine metabolism, in rumen microbiome, affected by gastrointestinal nematodes infection in lambs. (AU)

• FAPESP's process: 18/06191-6 - Transcriptome on lambs testicles from ewe fed cottonseed
• Grantee: Helder Louvandini
• Support Opportunities: Scholarships abroad - Research