| Full text | |
| Author(s): Show less - |
Rego, Gabriel N. A.
[1]
;
Nucci, Mariana P.
[2]
;
Mamani, Javier B.
[1]
;
Oliveira, Fernando A.
[1]
;
Marti, Luciana C.
[1]
;
Filgueiras, Igor S.
[1]
;
Ferreira, Joao M.
[1]
;
Real, Caroline C.
[3]
;
Faria, Daniele de Paula
[3]
;
Espinha, Paloma L.
[1]
;
Fantacini, Daianne M. C.
[4]
;
Souza, Lucas E. B.
[4]
;
Covas, Dimas T.
[4]
;
Buchpiguel, Carlos A.
[3]
;
Gamarra, Lionel F.
[1]
Total Authors: 15
|
| Affiliation: | [1] Hosp Israelita Albert Einstein, BR-05652900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med, Lab Magnet Resonance LIM 44, BR-01246903 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med, Dept Radiol & Oncol, Lab Nucl Med LIM 43, BR-01246903 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-4049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 3 FEB 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Magnetic hyperthermia (MHT) has been shown as a promising alternative therapy for glioblastoma (GBM) treatment. This study consists of three parts: The first part evaluates the heating potential of aminosilane-coated superparamagnetic iron oxide nanoparticles (SPIONa). The second and third parts comprise the evaluation ofMHT multiple applications in GBM model, either in vitro or in vivo. The obtained heating curves of SPIONa (100 nm, +20 mV) and their specific absorption rates (SAR) stablished the best therapeutic conditions for frequencies (309 kHz and 557 kHz) and magnetic field (300 Gauss), which were stablished based on three in vitro MHT application in C6 GBM cell line. The bioluminescence (BLI) signal decayed in all applications and parameters tested and 309 kHz with 300 Gauss have shown to provide the best therapeutic effect. These parameters were also established for three MHT applications in vivo, in which the decay of BLI signal correlates with reduced tumor and also with decreased tumor glucose uptake assessed by positron emission tomography (PET) images. The behavior assessment showed a slight improvement after each MHT therapy, but after three applications the motor function displayed a relevant and progressive improvement until the latest evaluation. Thus, MHT multiple applications allowed an almost total regression of the GBM tumor in vivo. However, futher evaluations after the therapy acute phase are necessary to follow the evolution or tumor total regression. BLI, positron emission tomography (PET), and spontaneous locomotion evaluation techniques were effective in longitudinally monitoring the therapeutic effects of the MHT technique. (AU) | |
| FAPESP's process: | 16/21470-3 - Therapeutic action of mesenchymal stem cells from human bone marrow labeled with multimodal nanoparticles in diabetic rats subjected to stroke: study of cellular, molecular and functional mechanisms. |
| Grantee: | Lionel Fernel Gamarra Contreras |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50983-3 - INCT 2014: complex fluids |
| Grantee: | Antonio Martins Figueiredo Neto |
| Support Opportunities: | Research Projects - Thematic Grants |