Non-invasive Transdermal Delivery of Human Insulin Using Ionic Liquids: In vitro Studies

Author(s):Show less -

Jorge, Ludmilla R. ^[1] ; Harada, Liliam K. ^[1] ; Silva, Erica C. ^[1] ; Campos, Welida F. ^[1] ; Morelli, Fernanda C. ^[1] ; Shimamoto, Gustavo ^[2] ; Pereira, Jorge F. B. ^[3] ; Oliveira Jr, Jose M. ; Tubino, Matthieu ^[2] ; Vila, Marta M. D. C. ^[4] ; Balcao, Victor M. ^{[5, 6, 4]}

Affiliation

^[1] Univ Sorocaba, Lab Biofilms & Bacteriophages, PhageLab, Sorocaba - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas - Brazil

^[3] Univ Coimbra, CIEPOPF Dept Chem Engn, Coimbra - Portugal

^[4] Oliveira Jr, Jr., Jose M., Univ Sorocaba, Lab Biofilms & Bacteriophages, PhageLab, Sorocaba - Brazil

^[5] Univ Aveiro, Dept Biol, Aveiro - Portugal

^[6] Univ Aveiro, CESAM, Aveiro - Portugal

• Document type: Journal article
• Source: FRONTIERS IN PHARMACOLOGY; v. 11, APR 23 2020.
• Web of Science Citations: 0

Abstract

In this research project, synthesis and characterization of ionic liquids and their subsequent utilization as facilitators of transdermal delivery of human insulin was pursued. Choline geranate and choline oleate ionic liquids (and their deep eutectic solvents) were produced and characterized by nuclear magnetic resonance (H-1 NMR), water content, oxidative stability, cytotoxicity and genotoxicity assays, and ability to promote transdermal protein permeation. The results gathered clearly suggest that all ionic liquids were able to promote/facilitate transdermal permeation of insulin, although to various extents. In particular, choline geranate 1:2 combined with its virtually nil cyto- and geno-toxicity was chosen to be incorporated in a biopolymeric formulation making it a suitable facilitator aiming at transdermal delivery of insulin. (AU)

• FAPESP's process: 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles
• Grantee: Vitor Manuel Cardoso Figueiredo Balcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative
• Grantee: Vitor Manuel Cardoso Figueiredo Balcão
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids.
• Grantee: Marta Maria Duarte Carvalho Vila
• Support Opportunities: Regular Research Grants