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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differences in Cystic Fibrosis-Associated Burkholderia spp. Bacteria Metabolomes after Exposure to the Antibiotic Trimethoprim

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Author(s):
McAvoy, Andrew C. [1] ; Jaiyesimi, Olakunle [1] ; Threatt, Paxton H. [1] ; Seladi, Tyler [1] ; Goldberg, Joanna B. [2, 3] ; da Silva, Ricardo R. [4] ; Garg, Neha [5, 6, 2]
Total Authors: 7
Affiliation:
[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 - USA
[2] Emory Univ, Dept Pediat, Div Pulmonol Allergy Immunol Cyst Fibrosis & Slee, Sch Med, Atlanta, GA 30322 - USA
[3] Emory Childrens Cyst Fibrosis Ctr, Atlanta, GA 30322 - USA
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[5] Georgia Inst Technol, Sch Chem & Biochem, Sch Biol Sci, Ctr Microbial Dynam & Infect, Atlanta, GA 30332 - USA
[6] Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 - USA
Total Affiliations: 6
Document type: Journal article
Source: ACS INFECTIOUS DISEASES; v. 6, n. 5, p. 1154-1168, MAY 8 2020.
Web of Science Citations: 0
Abstract

The Burkholderia cepacia complex is a group of closely related bacterial species with large genomes that infect immunocompromised individuals and those living with cystic fibrosis. Some of these species are found more frequently and cause more severe disease than others, yet metabolomic differences between these have not been described. Furthermore, our understanding of how these species respond to antibiotics is limited. We investigated the metabolomics differences between three most prevalent Burkholderia spp. associated with cystic fibrosis: B. cenocepacia, B. multivorans, and B. dolosa in the presence and absence of the antibiotic trimethoprim. Using a combination of supervised and unsupervised metabolomics data visualization and analysis tools, we describe the overall differences between strains of the same species and between species. Specifically, we report, for the first time, the role of the pyomelanin pathway in the metabolism of trimethoprim. We also report differences in the detection of known secondary metabolites such as fragin, ornibactin, and N-acylhomoserine lactones and their analogs in closely related strains. Furthermore, we highlight the potential for the discovery of new secondary metabolites in clinical strains of Burkholderia spp. The metabolomics differences described in this study highlight the personalized nature of closely related Burkholderia strains. (AU)

FAPESP's process: 17/18922-2 - Development of a computing platform extensible and modular for metabolomics and metagenomics analysis: innovation with the discovery of new enzymatic activities and natural products of pharmaceutical interest derived
Grantee:Ricardo Roberto da Silva
Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants
FAPESP's process: 19/05026-4 - Development of a computational platform extensible and modular for analysis of metabolomics and metagenomics experiments: Innovating with the discovery of new enzymatic activities and derived natural products of interest
Grantee:Ricardo Roberto da Silva
Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers