| Full text | |
| Author(s): Show less - |
Antunes, Geiza Fernanda
[1]
;
Gouveia, Flavia Venetucci
[1, 2]
;
Rezende, Fabiana Strambio
[1, 2]
;
de Jesus Seno, Midia Dias
[1, 2]
;
de Carvalho, Milene Cristina
[3, 4]
;
de Oliveira, Caroline Cruz
[1, 2]
;
Tosati dos Santos, Lennon Cardoso
[1, 2]
;
de Castro, Marina Correia
[1, 2]
;
Kuroki, Mayra Akemi
[1, 2]
;
Teixeira, Manoel Jacobsen
[5]
;
Otoch, Jose Pinhata
[6]
;
Brandao, Marcus Lira
[3, 4]
;
Fonoff, Erich Talamoni
[5]
;
Ruiz Martinez, Raquel Chacon
[1, 2, 7, 8]
Total Authors: 14
|
| Affiliation: | [1] Hosp Sirio Libanes, Div Neurosci, Sao Paulo - Brazil
[2] Ruiz Martinez, Raquel Chacon, Univ Cidade Sao Paulo, UNICID, Sao Paulo, Brazil.Antunes, Geiza Fernanda, Hosp Sirio Libanes, Div Neurosci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Campus USP, Ribeirao Preto - Brazil
[4] Inst Neurosci & Behav INeC, Campus USP, Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Inst Psychiat, Dept Neurol, Div Funct Neurosurg, Sch Med, Sao Paulo - Brazil
[6] Univ Sao Paulo, Sch Med, Dept Surg Tech, Sao Paulo - Brazil
[7] Univ Sao Paulo, Inst Psychiat, LIM 23, Lab Psicopatol & Terapeut Psiquiatr, Sch Med, Sao Paulo - Brazil
[8] Univ Cidade Sao Paulo, UNICID, Sao Paulo - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | NEUROBIOLOGY OF STRESS; v. 12, MAY 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Avoidance behavior is a hallmark in pathological anxiety disorders and results in impairment of daily activities. Individual differences in avoidance responses are critical in determining vulnerability or resistance to anxiety disorders. Dopaminergic activation is implicated in the processing of avoidance responses; however, the mechanisms underlying these responses are unknown. In this sense, we used a preclinical model of avoidance behavior to investigate the possibility of an intrinsic differential dopaminergic pattern between good and poor performers. The specific goal was to assess the participation of dopamine (DA) through pharmacological manipulation, and we further evaluated the effects of systemic injections of the dopaminergic receptor type 1 (D1 antagonist - SCH23390) and dopaminergic receptor type 2 (D2 antagonist - sulpiride) antagonists in the good performers. Additionally, we evaluated the effects of intra-amygdala microinjection of a D1 antagonist (SCH23390) and a D2 antagonist (sulpiride) in good performers as well as intra-amygdala microinjection of a D1 agonist (SKF38393) and D2 agonist (quinpirole) in poor performers. Furthermore, we quantified the contents of dopamine and metabolites (3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA)) in the amygdala, evaluated the basal levels of tyrosine hydroxylase expression (catecholamine synthesis enzyme) and measured the volume of the substantia nigra, ventral tegmental area and locus coeruleus. Our results showed that it could be possible to convert animals from good to poor performers, and vice versa, by intra-amygdala (basolateral and central nucleus) injections of D1 receptor antagonists in good performers or D2 receptor agonists in poor performers. Additionally, the good performers had lower levels of DOPAC and HVA in the amygdala, an increase in the total volume of the amygdala (AMG), substantia nigra (SN), ventral tegmental area (VTA) and locus coeruleus (LC), and an increase in the number of tyrosine hydroxylase-positive cells in SN, VTA and LC, which positively correlates with the avoidance behavior. Taken together, our data show evidence for a dopaminergic signature of avoidance performers, emphasizing the role of distinct dopaminergic receptors in individual differences in avoidance behavior based on pharmacological, immunohistochemical, neurochemical and volumetric analyses. Our findings provide a better understanding of the role of the dopaminergic system in the execution of avoidance behavior. (AU) | |
| FAPESP's process: | 17/10466-8 - Towards revealing aggression: imaging the aggressive brain |
| Grantee: | Flavia Venetucci Gouveia |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/20602-5 - Prospective analyses of the functional results of philanthropist bilateral amygdalectomy in patients with refractory aggression |
| Grantee: | Flavia Venetucci Gouveia |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 11/08575-7 - The role of dopaminergic modulation in the lateral nucleus of amygdala during active avoidance response |
| Grantee: | Raquel Chacon Ruiz Martinez |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 14/12999-5 - Lineage factor evaluation of aversive learning test anxiety levels in good and bad performance animals: Wistar versus Sprague Dawley |
| Grantee: | Lennon Tosati Cardoso Dos Santos |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |