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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

M-CSF- and L929-derived macrophages present distinct metabolic profiles with similar inflammatory outcomes

Full text
Author(s):
de Freitas, Leonardo Pimentel [1]
Total Authors: 1
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Lab Immunometab, Rua Monteiro Lobato 255, Bloco H, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Immunobiology; v. 225, n. 3 MAY 2020.
Web of Science Citations: 0
Abstract

Macrophages are essential components of the immune system. Macrophages can be derived from the bone marrow of mice with either recombinant M-CSF or L929 supernatant. Recent literature considers recombinant M-CSF- and L929-derived macrophages as equals, even though L929-derived macrophages are exposed to other substances secreted in the L929 supernatant, and not only M-CSF. Thus, we decided to perform a comparative analysis of both inflammatory and metabolic profiles of macrophages differentiated under the aforementioned conditions, which is relevant for standardization and interpretation of in vitro studies. We observed that, when treated with LPS, L929macs secrete lower levels of proinflammatory cytokines (TNF-alpha, IL-6, IL12) and present higher glycolysis and oxygen consumption when compared with M-CSFmacs. L929macs also have increased mitochondrial mass, with higher percentage of dysfunctional mitochondria. This sort of information can help direct further studies towards a more specific approach for macrophage generation. (AU)

FAPESP's process: 16/18031-8 - HIF-1 alpha in metabolic and functional control of adipose tissue resident macrophages in diabetes induced by obesity
Grantee:Gustavo Gastão Davanzo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/15626-8 - Macrophages and T lymphocytes immunometabolism in metabolic and inflammatory diseases
Grantee:Pedro Manoel Mendes de Moraes Vieira
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/23328-0 - Study of the metabolic regulation of macrophages by leptin
Grantee:Lauar de Brito Monteiro
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 19/06372-3 - Multiparametric flow cytometry
Grantee:Alessandro dos Santos Farias
Support Opportunities: Multi-user Equipment Program