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Multiparametric flow cytometry

Grant number: 19/06372-3
Support type:Multi-user Equipment Program
Duration: August 01, 2019 - July 31, 2026
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Principal researcher:Alessandro dos Santos Farias
Grantee:Alessandro dos Santos Farias
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers:José Luiz Proença Módena ; Marcelo Alves da Silva Mori ; Marco Aurélio Ramirez Vinolo ; Pedro Manoel Mendes de Moraes Vieira
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment:Processos Biológicos - Caracterização - Contagem células (inclui citômetros)
Manufacturer: Fabricante não informado
Model: Modelo não informado


Flow cytometry is a technology that provides the analysis of physical and chemical characteristics of a particular suspended particle. Flow cytometry allows the detection of multiple simultaneous characteristics (size, complexity and fluorescence intensity) of an individualized particle or cell. After the advent of monoclonal antibodies, the main application of flow cytometry was the identification of cell populations and subpopulations through surface and intracellular factors. In the last decades, several technologies were developed providing other applications for flow cytometry, which include the determination of solutes, identification of cell cycle phases, apoptosis, cell proliferation, identification of bacterial and viral particles, expression and protein phosphorylation (PhosFlow) and identification and quantification of gene expression (PrimeFlow). More recently, the development of equipments capable of analyzing simultaneously several parameters (> 20) provided a new era in flow cytometry. Today, multiparametric cytometry is the state of the art in immunology research. This technology has enabled the identification of new cellular populations. The possibility of analyzing up to 50 parameters in a single cell not only alters the discriminatory capacity of cellular subtypes, but also provides greater data reliability and faster data generation. Not least, the increased analytical capacity of a single experiment provides considerable savings in reagents and materials, which optimize the expense of resources. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FACHI, J. L.; PRAL, L. P.; DOS SANTOS, J. A. C.; CODO, A. C.; DE OLIVEIRA, S.; FELIPE, J. S.; ZAMBOM, F. F. F.; CAMARA, N. O. S.; VIEIRA, P. M. M. M.; COLONNA, M.; VINOLO, M. A. R. Hypoxia enhances ILC3 responses through HIF-1 alpha-dependent mechanism. MUCOSAL IMMUNOLOGY, JAN 2021. Web of Science Citations: 0.
BRANDAO, W. N.; DE OLIVEIRA, M. G.; ANDREONI, R. T.; NAKAYA, H.; FARIAS, A. S.; PERON, J. P. S. Neuroinflammation at single cell level: What is new?. Journal of Leukocyte Biology, AUG 2020. Web of Science Citations: 0.
DE FREITAS, LEONARDO PIMENTEL. M-CSF- and L929-derived macrophages present distinct metabolic profiles with similar inflammatory outcomes. Immunobiology, v. 225, n. 3 MAY 2020. Web of Science Citations: 0.
BOLDRINI, VINICIUS O.; BRANDAO, CARLOS OTAVIO; PIMENTEL, V, MARIA LUCIA; VIDAL, ALINE; MANSUR, LETICIA F.; QUINTILIANO, RAPHAEL P. S.; SANTOS, LEONILDA M. B.; FARIAS, ALESSANDRO S. Massive activity of cytotoxic cells during refractory Neuromyelitis Optica spectrum disorder. Journal of Neuroimmunology, v. 340, MAR 15 2020. Web of Science Citations: 0.
PERON, J. P. S.; NAKAYA, I, H.; SCHLINDWEIN, M. A. M.; GONCALVES, M. V. M. COVID-19 Pandemic and Dysbiosis: Can the Ivermectin Hysteria Lead to an Increase of Autoimmune Neuroinflammatory Diseases?. CRITICAL REVIEWS IN IMMUNOLOGY, v. 40, n. 6, SI, p. 537-542, 2020. Web of Science Citations: 0.

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