Modulation of the intestinal microbiota and the metabolites produced by the administration of ice cream and a dietary supplement containing the same probiotics

Author(s)

Rodrigues, Vivian Cristina da Cruz ^[1] ; Duque, Ana Luiza Rocha Faria ^[2] ; Fino, Luciana de Carvalho ^{[1, 3]} ; Simabuco, Fernando Moreira ^{[1, 3]} ; Sartoratto, Adilson ^[4] ; Cabral, Lucelia ^[5] ; Noronha, Melline Fontes ^[6] ; Sivieri, Katia ^[2] ; Antunes, Adriane Elisabete Costa ^{[1, 3]}

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci FCA, BR-13484350 Limeira, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Food & Nutr, BR-14800903 Araraquara, SP - Brazil

^[3] Noronha, Melline Fontes, Univ Illinois, Res Resource Ctr, Res Informat Core, Genome Res Div, Chicago, IL USA.Rodrigues, Vivian Cristina da Cruz, Univ Estadual Campinas, Sch Appl Sci FCA, BR-13484350 Limeira, SP - Brazil

^[4] Univ Estadual Campinas, Pluridisciplinary Ctr Chem Biol & Agr Res CPQBA, Div Organ & Pharmaceut Chem, BR-13148218 Paulinia, SP - Brazil

^[5] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, BR-13083970 Campinas, SP - Brazil

^[6] Univ Illinois, Res Resource Ctr, Res Informat Core, Genome Res Div, Chicago, IL - USA

• Document type: Journal article
• Source: BRITISH JOURNAL OF NUTRITION; v. 124, n. 1, p. 57-68, JUL 14 2020.
• Web of Science Citations: 5

Abstract

The aim of the present work was to compare the capacity to modulate the intestinal microbiota and the production of metabolites after 14 d administration of a commercial dietary supplement and a manufactured ice cream, both containing the same quantity of inulin and the same viable counts of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis BB-12, using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R)) model. Samples of the colonic contents were evaluated microbiologically by real-time quantitative PCR (qRT-PCR) and next-generation sequencing and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions (NHthorn 4). Statistical analyses were carried out for all the variables using the two-way ANOVA followed by the Tukey multiple comparisons test (P < 0.05) for metabolite production, qRT-PCR and the bioinformatics analysis for microbiota diversity. Dietary supplement and ice cream were able to deliver the probiotic L. acidophilus and B. animalis to the simulated colon and modulate the microbiota, increasing beneficial micro-organisms such as Bifidobacterium spp., Bacteroides spp. and Faecalibacterium spp. for dietary supplement administration, and Lactobacillus spp. for ice cream supplementation. However, the ice cream matrix was probably more favourable for the maintenance of the metabolic activity of the probiotics in the SHIME (R) model, due to the larger amounts of acetate, propionate, butyrate and ammonium ions obtained after 14 d of supplementation. In conclusion, both ways of probiotic supplementation could be efficient, each with its own particularities. (AU)

• FAPESP's process: 17/25007-9 - MATRIX EFFECT ON VEHICULATION AND FUNCTIONALITY OF PRE AND PROBIOTICS: STUDY IN A DYNAMIC COLONIC MODEL AND WITH VOLUNTEERS FROM THE PREPARATORY SCHOOL OF ARMY CADETS FROM CAMPINAS
• Grantee: Adriane Elisabete Antunes de Moraes
• Support Opportunities: Regular Research Grants