An electrochemical immunosensor using gold nanoparticles-PAMAM-nanostructured screen-printed carbon electrodes for tau protein determination in plasma and brain tissues from Alzheimer patients

Author(s):Show less -

Razzino, Claudia A. ^{[1, 2]} ; Serafin, Veronica ^{[1, 3]} ; Gamella, Maria ^{[1, 3]} ; Pedrero, Maria ^{[1, 3]} ; Montero-Calle, Ana ^[4] ; Barderas, Rodrigo ^[4] ; Calero, Miguel ^[5] ; Lobo, Anderson O. ^[6] ; Yanez-Sedeno, Paloma ^{[1, 3]} ; Campuzano, Susana ^{[1, 3]} ; Pingarron, Jose M. ^{[1, 3]}

Affiliation

^[1] Univ Complutense Madrid, Fac Chem Sci, Dept Analyt Chem, Madrid 28040 - Spain

^[2] Univ Vale Paraiba, Inst Res & Dev, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[3] Lobo, Anderson O., Univ Fed Piaui, Dept Mat Engn, LIMAV Interdisciplinary Lab Adv Mat, BioMatLab, BR-64049550 Teresina, PI, Brazil.Razzino, Claudia A., Univ Complutense Madrid, Fac Chem Sci, Dept Analyt Chem, Madrid 28040 - Spain

^[4] Carlos III Hlth Inst, UFIEC, Chron Dis Programme, Madrid 28220 - Spain

^[5] Carlos III Inst Hlth, Alzheimers Ctr, CIEN Fdn & CIBERNED, Reina Sofia Fdn, Madrid 28220 - Spain

^[6] Univ Fed Piaui, Dept Mat Engn, LIMAV Interdisciplinary Lab Adv Mat, BioMatLab, BR-64049550 Teresina, PI - Brazil

• Document type: Journal article
• Source: BIOSENSORS & BIOELECTRONICS; v. 163, SEP 1 2020.
• Web of Science Citations: 0

Abstract

This work reports a new sensitive strategy for the determination of tau protein, a hallmark of Alzheimer's disease (AD), involving a sandwich immunoassay and ampemmetric detection at disposable screen-printed carbon electrodes (SPCEs) modified with a gold nanoparticles-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM) covalently immobilized onto electrografted p-aminobenzoic acid (p-ABA). The capture antibody (CAb) was immobilized by crosslinking with glutaraldehyde (GA) on the amino groups of the 3D-Au-PAMAM-p-ABA-SPCE, where tau protein was sandwiched with a secondary antibody labeled with horseradish peroxidase (HRP-DAb). Amperometry at -200 mV (vs the Ag pseudo-reference electrode) upon the addition of hydroquinone (HQ) as electron transfer mediator and H2O2 as the enzyme substrate was used to detect the immunocomplex formation. The great analytical performance of the immunosensor in terms of selectivity and low limit of detection (LOD) (1.7 pg mL(-1)) allowed the direct determination of the target protein in raw plasma samples and in brain tissue extracts from healthy individuals and post mortem diagnosed AD patients, using a simple and fast protocol. (AU)

• FAPESP's process: 18/14130-7 - Dendrimer-encapsulated nanoparticles as a sensing platform for Alzheimer´s biomarker detection
• Grantee: Claudia Do Amaral Razzino
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor