Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study

Author(s)

Caroline Loureiro ^[1] ; Marília Afonso Rabelo Buzalaf ^[2] ; Juliano Pelim Pessan ^[3] ; Felipe Ricardo Nunes de Moraes ^[4] ; Vinícius Taioqui Pelá ^[5] ; Talita Mendes Oliveira Ventura ^[6] ; Rogério de Castilho Jacinto ^[7]

Affiliation

^[1] Universidade Estadual Paulista. Araçatuba School of Dentistry. Department of Preventive and Restorative Dentistry - Brasil

^[2] Universidade de São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[3] Universidade Estadual Paulista. Araçatuba School of Dentistry. Department of Preventive and Restorative Dentistry - Brasil

^[4] Universidade Estadual Paulista. Araçatuba School of Dentistry. Department of Preventive and Restorative Dentistry - Brasil

^[5] Universidade Federal de São Carlos. Department of Genetics and Evolution - Brasil

^[6] Universidade Estadual Paulista. Araçatuba School of Dentistry. Department of Preventive and Restorative Dentistry - Brasil

^[7] Universidade Estadual Paulista. Araçatuba School of Dentistry. Department of Preventive and Restorative Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 31, n. 3, p. 319-336, 2020-07-13.

Abstract

Abstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions. (AU)

• FAPESP's process: 18/08282-9 - Proteomic profile of bacteria present in root canals of teeth with symptomatic or asymptomatic apical periodontitis
• Grantee: Felipe Ricardo Nunes de Moraes
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/14995-0 - Proteomic profile of endodontic infections in diabetic patients
• Grantee: Caroline Loureiro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/18741-0 - Proteomic profile of bacteria from root canals of symptomatic or asymptomatic teeth with apical periodontitis
• Grantee: Rogerio de Castilho Jacinto
• Support Opportunities: Regular Research Grants