Outbreaks ofBemisia tabaciMediterranean species in vegetable crops in Sao Paulo and Parana States, Brazil

Author(s):Show less -

Bello, Vinicius Henrique ^[1] ; Watanabe, Luis Fernando Maranho ^[1] ; Fusco, Lucas Machado ^[1] ; De Marchi, Bruno Rossitto ^[1] ; da Silva, Felipe Barreto ^[1] ; Gorayeb, Eduardo Silva ^[1] ; Moura, Monika Fecury ^[1] ; de Souza, Isabela Morcilo ^[1] ; Muller, Cristiane ^[2] ; Salas, Fernando Javier Sanhueza ^[3] ; Yuki, Valdir Atsushi ^[4] ; Bueno, Regiane Cristina Oliveira de Freitas ^[1] ; Pavan, Marcelo Agenor ^[1] ; Krause-Sakate, Renate ^[1]

Affiliation

^[1] UNESP, Fac Ciencias Agron, Botucatu, SP - Brazil

^[2] Corteva Agrisci, Mogi Mirim - Brazil

^[3] Inst Biol, Sao Paulo - Brazil

^[4] Inst Agron Estado Sao Paulo, Campinas - Brazil

• Document type: Journal article
• Source: BULLETIN OF ENTOMOLOGICAL RESEARCH; v. 110, n. 4, p. 487-496, AUG 2020.
• Web of Science Citations: 1

Abstract

The whitefly,Bemisia tabaci(Gennadius) (Hemiptera: Aleyrodidae), is one of the most important agricultural pests and virus vectors worldwide.Bemisia tabaciis considered a complex of cryptic species with at least 44 species. Among them, the species Middle East-Asia Minor 1 (MEAM1, formerly B biotype) and Mediterranean (MED, formerly Q biotype) are the most important, and they have attained global status. In Brazil, MEAM1 was first reported in the 1990s and is currently the predominant species in the country, meanwhile, MED was recently reported in the South and Southeast regions and was found to be mainly associated with ornamental plants. Currently, an increasing problem in the management of whitefly infestations in greenhouses associated with bell pepper was observed in Sao Paulo State, Brazil. The whiteflies were collected and identified based on a microsatellite locus (primer pair BEM23F and BEM23R) and the mitochondrial cytochrome oxidase I gene followed by restriction fragment length polymorphism analysis and sequencing. We observed that MED was the predominant species collected on bell pepper, but it was also found on tomato, cucumber, eggplant, and weeds grown in greenhouses. In open field, we found MED on tomatoes, bell peppers, and eggplants. In addition, MED was identified in Goias State in association with ornamental plants. The begomovirusTomato severe rugose virusand the crinivirusTomato chlorosis viruswas detected on bell pepper and tomato, respectively. Only MED specimens were found associated with the virus-infected plants. Moreover, we also investigated the endosymbionts present in the MED whiteflies. The collected populations ofB. tabaciMED harbored a diversity of secondary endosymbionts, withHamiltonella(H) found predominantly in 89 specimens of the 129 tested. These results represent a new concern for Brazilian agriculture, especially for the management of the newly introduced whitefly MED species, which must be implemented to limit the spreading and establishment of this pest in different crops in this country. (AU)

• FAPESP's process: 17/21588-7 - Bemisia tabaci in Brazil: competitive displacement between criptic species, susceptibility to insecticides, next generation sequencing analysis, virus influence on insect biology and the presence of the endosymbiont Hamiltonella in virus transmission
• Grantee: Renate Krause Sakate
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18274-3 - Begomovirus and crinivirus in solanasceous: regional molecular epidemiology and sustainable integrated management alternatives
• Grantee: Jorge Alberto Marques Rezende
• Support Opportunities: Research Projects - Thematic Grants