Expression of tissue factor mRNA in thrombosis associated with antiphospholipid syndrome

Author(s):Show less -

Mazetto, Bruna de Moraes ^[1] ; Lazarini, Mariana ^[2] ; Tobaldini, Lais Quinteiro ^{[2, 1]} ; Arantes, Fernanda Talge ^{[2, 1]} ; dos Santos, Ana Paula Rosa ^{[2, 1]} ; Jacinto, Bruna Cardoso ^{[2, 1]} ; Vaz, Camila de Oliveira ^{[2, 1]} ; Mesquita, Gabriela Tripiquia Vechiatto ^{[2, 1]} ; Saraiva, Sabrina da Silva ^{[2, 1]} ; Annichino-Bizzacchi, Joyce ^{[2, 3]} ; Orsi, Fernanda Andrade ^{[2, 4]}

Affiliation

^[1] Univ Estadual Campinas, Fac Med Sci, Campinas - Brazil

^[2] Univ Estadual Campinas, Hematol & Hemotherapy Ctr, Campinas - Brazil

^[3] Univ Estadual Campinas, Dept Clin Med, Campinas - Brazil

^[4] Univ Estadual Campinas, Dept Clin Pathol, Fac Med Sci, Campinas R Tessalia Vieira de Camargo 126, BR-13083887 Campinas - Brazil

• Document type: Journal article
• Source: JOURNAL OF THROMBOSIS AND THROMBOLYSIS; v. 51, n. 2 JUL 2020.
• Web of Science Citations: 0

Abstract

Tissue factor (TF) is a procoagulant protein associated with increased risk of thrombotic events in antiphospholipid syndrome (t-APS). The mechanisms by which TF levels are increased in APS have not yet been established. The aim of this study was to investigate whetherTFmRNA expression is associated with TF levels and thrombosis in APS. We compared levels of circulating TF and high sensitivity C-reactive protein (hs-CRP) between t-APS and controls (individuals without thrombosis). The association betweenTFmRNA expression, quantified by real time quantitative polymerase chain reaction, and t-APS was accessed using regression analysis. We included 41 controls and 42 t-APS patients, mean age was 41 years old (SD 14) in both groups. Hs-CRP and TF levels were higher in t-APS patients (mean hs-CRP levels 0.81 mg/dL {[}SD 1.88] and median TF levels 249.0 pg/mL {[}IQR 138.77-447.61]) as compared to controls (mean hs-CRP levels 0.24 mg/dL {[}SD 0.26] and median TF levels 113.0 pg/mL {[}IQR 81.17-161.53];P = 0.02 andP < 0.0001, respectively). There was no correlation betweenTFmRNA expression and TF levels in t-APS (r - 0.209,P = 0.19).TFmRNA expression was not associated with t-APS (adjusted OR 1.16; 95%CI 0.72-1.87). Despite circulating TF levels being higher in patients with t-APS than in controls,TFmRNA expression was similar between groups. The results demonstrate thatTFmRNA expression is not associated with levels of circulating TF and hypercoagulability in t-APS. (AU)

• FAPESP's process: 16/14172-6 - Investigation of the pathophysiological aspects and novel therapeutic approaches for thromboembolic disorders
• Grantee: Joyce Maria Annichino-Bizzacchi
• Support Opportunities: Research Projects - Thematic Grants