Single nucleotide polymorphism rs4284505 in microRNA17 and risk of dental fluorosis

Author(s)

Abbasoglu, Zerrin ^[1] ; Dalledone, Mariana ^[2] ; Wambier, Leticia M. ^[3] ; Pecharki, Giovana ^[4] ; Baratto-Filho, Flares ^{[2, 5]} ; Andrades, Kesly Mary R. ^[5] ; Scariot, Rafaela ^[2] ; Trevilatto, Paula C. ^[6] ; Brancher, Joao A. ^[2] ; Kuchler, Erika C. ^{[2, 5]}

Affiliation

^[1] Yeditepe Univ, Dept Pediat Dent, Istanbul - Turkey

^[2] Univ Positivo, Sch Hlth & Biol Sci, R Prof Pedro Viriato Parigot de Souza, BR-5300 Curitiba, Parana - Brazil

^[3] Univ Positivo, Sch Hlth & Biol Sci, Dept Dent, Curitiba, Parana - Brazil

^[4] Univ Fed Parana, Dept Community Hlth, Curitiba, Parana - Brazil

^[5] Univille Univ, Sch Dent, Joinville - Brazil

^[6] Pontificia Univ Catolica Parana PUC PR, Dent Sch, Curitiba, Parana - Brazil

• Document type: Journal article
• Source: ACTA ODONTOLOGICA SCANDINAVICA; v. 78, n. 6 JUL 2020.
• Web of Science Citations: 0

Abstract

Objectives:The aim of this study is to evaluate the association between the single nucleotide polymorphism (SNP) rs4284505 within the gene that codifies microRNA17 (miRNA17) and dental fluorosis (DF) in a group of children. Methods:Children living in a city with fluoridation of public water supplies were included. DF was assessed in erupted permanent teeth by Dean's modified index. The miR-SNP rs4284505 was selected in miRNA17 and genotyping was carried out by real-time PCR. Genotype and allelic distributions between DF and control, and between DF phenotypes (mild, moderate and severe) and control were analysed. Results:Among a total of 527 children enrolled for the study, 383 were DF free and 144 presented DF. In the dominant model analysis (AA + AGvs.GG) the miR-SNP rs4284505 was associated with moderate DF, with carriers of the GG genotype having an increased risk of more than two times for DF (p = 0.031; Odds Ratio = 2.26, Confidence Interval 95%= 1.04-4.73). Allelic distribution showed borderline statistical significance for moderate DF with the carriers of G allele having an increased risk for DF (p = .050; Odds Ratio = 1.75, Confidence Interval 95%= 1.00-3.12). Conclusion:The miR-SNP rs4284505 in miRNA17 was associated with an increased risk of DF. (AU)

• FAPESP's process: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/08149-1 - Evaluation of the role of estrogen in dentofacial tissues
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Scholarships in Brazil - Young Researchers