Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model

Author(s)

Leite, Marcel Nani ^{[1, 2]} ; Leite, Saulo Nani ^{[1, 3]} ; Caetano, Guilherme Ferreira ^{[1, 2, 4]} ; Moretti de Andrade, Thiago Antonio ^{[1, 2, 4]} ; Fronza, Marcio ^{[1, 5]} ; Cipriani Frade, Marco Andrey ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Ribeirao Preto, SP - Brazil

^[3] Fundacao Educ Guaxupe, Dept Physiotherapy, Guaxupe, MG - Brazil

^[4] Ctr Univ Fundacao Herminio Ometto, Grad Program Biomed Sci, Araras, SP - Brazil

^[5] Univ Vila Velha, Dept Pharm, Grad Program Pharmaceut Sci, Vila Velha, ES - Brazil

• Document type: Journal article
• Source: ANAIS BRASILEIROS DE DERMATOLOGIA; v. 95, n. 4, p. 418-427, JUL-AUG 2020.
• Web of Science Citations: 0

Abstract

Background: Dermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. Objective: This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. Methods: For in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. Results: Latex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitations: Acute superficial wound model in rats and non-use of gel-cream (medium) without latex. Conclusion: In conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline. (C) 2020 Sociedade Brasileira de Dermatologia. Published by Elsevier Espana, S.L.U. (AU)

• FAPESP's process: 14/23662-1 - Production of 3D scaffolds containing different concentrations of graphene, hydroxyapatite and tricalcium phosphate surface coated with human bone morphogenetic and P-1 latex proteins
• Grantee: Guilherme Ferreira Caetano
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate