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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Viral metagenomics performed in patients with acute febrile syndrome during Toxoplasma gondii outbreak in south Brazil

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Author(s):
Bezerra, Rafael dos Santos [1, 2] ; Diefenbach, Cristiane Fracao [3] ; Pereira, Dalnei Veiga [3] ; Kashima, Simone [2] ; Slavov, Svetoslav Nanev [2, 4]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Ciencias Biol & Saude, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Hemoctr, Ribeirao Preto, SP - Brazil
[3] Hosp Caridade Dr Astrogildo De Azevedo, Dept Hematol & Oncol, Santa Maria, RS - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med Interna, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Infectious Diseases; v. 24, n. 3, p. 250-255, MAY-JUN 2020.
Web of Science Citations: 0
Abstract

Toxoplasmosis is a zoonotic infection caused by the protozoan parasite Toxoplasma gondii. The infection is widely disseminated in the human population and is usually benign or asymptomatic. Systemic T. gondii infection presents risks for pregnant women and AIDS patients. Although rare, T. gondii can cause outbreaks in urban centers. The origin of these outbreaks is not completely understood but probably results from introduction of zoonotic T. gondii strains in the population. During such outbreaks other pathogens which mimic T. gondii acute febrile syndrome may also circulate; therefore, detailed investigation of the outbreak is of extreme importance. In this study we performed viral metagenomics next generation sequencing (mNGS) in patient samples obtained during T. gondii outbreak in Santa Maria city, South Brazil. Specific bioinformatics pipelines specialized in virus discovery were applied in order to identify co-circulating vial agents. Epstein Barr virus and Parvovirus B19 contigs were assembled and these viruses can cause symptoms similar to toxoplasmosis. In conclusion, our findings show the importance of Metagenomics next generation sequencing (mNGS) use to help characterize the outbreak more completely and in the management of the affected patients. (C) 2020 Sociedade Brasileira de Infectologia. Published by Elsevier Espana, S.L.U. (AU)

FAPESP's process: 17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 19/07861-8 - Development of a bioinformatic pipeline for identification of emerging infectious diseases among patients in regimen of chronic transfusion
Grantee:Ian Nunes Valença
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/15826-5 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem-cell transplantation by multiple-research approach
Grantee:Svetoslav Nanev Slavov
Support type: Scholarships in Brazil - Young Researchers
FAPESP's process: 19/08528-0 - Application of bioinformatic procedures for identification of the impact of the viral infections in hemotherapy
Grantee:Rafael dos Santos Bezerra
Support type: Scholarships in Brazil - Master