| Full text | |
| Author(s): |
Polo, Carla C.
[1]
;
Pereira, Luciano
[2, 3]
;
Mazzafera, Paulo
[3, 4]
;
Flores-Borges, Denisele N. A.
[4]
;
Mayer, Juliana L. S.
[4]
;
Guizar-Sicairos, Manuel
[5]
;
Holler, Mirko
[5]
;
Barsi-Andreeta, Mariane
[6]
;
Westfahl Jr, Harry
;
Meneau, Florian
[7]
Total Authors: 10
|
| Affiliation: | [1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil
[2] Agron Inst IAC, Ctr R&D Ecophysiol & Biophys, Lab Plant Physiol Coaracy M Franco, Campinas, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Plant Biol, POB 6109, BR-13083970 Campinas, SP - Brazil
[4] Univ Sao Paulo ESALQ USP, Coll Agr Luiz de Queiroz, Dept Crop Sci, CP 09, BR-13418900 Piracicaba, SP - Brazil
[5] Paul Scherrer Inst, Villigen - Switzerland
[6] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil
[7] Westfahl Jr, Jr., Harry, Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 10, n. 1 APR 7 2020. |
| Web of Science Citations: | 3 |
| Abstract | |
Lignin is a heterogeneous aromatic polymer responsible for cell wall stiffness and protection from pathogen attack. However, lignin represents a bottleneck to biomass degradation due to its recalcitrance related to the natural cell wall resistance to release sugars for fermentation or further processing. A biological approach involving genetics and molecular biology was used to disrupt lignin pathway synthesis and decrease lignin deposition. Here, we imaged three-dimensional fragments of the petioles of wild type and C4H lignin mutant Arabidopsis thaliana plants by synchrotron cryoptychography. The three-dimensional images revealed the heterogeneity of vessels, parenchyma, and fibre cell wall morphologies, highlighting the relation between disturbed lignin deposition and vessel implosion (cell collapsing and obstruction of water flow). We introduce a new parameter to accurately define cell implosion conditions in plants, and we demonstrate how cryo-ptychographic X-ray computed tomography (cryo-PXCT) provides new insights for plant imaging in three dimensions to understand physiological processes. (AU) | |
| FAPESP's process: | 16/24249-6 - Coherent diffractive imaging applied to biological samples: assessment of 3D internal structure of vegetal fibers under different pretreatments |
| Grantee: | Carla Cristina Polo |
| Support Opportunities: | Scholarships abroad - Research |
| FAPESP's process: | 14/25964-5 - From the monomers to the nanoparticles: in situ real time investigation of gold nanoparticles formation by synchrotron and microscopy techniques |
| Grantee: | Florian Edouard Pierre Meneau |
| Support Opportunities: | Research Grants - Young Investigators Grants |