An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale

Author(s)

Brenelli, Livia B. ^{[1, 2]} ; Figueiredo, Fernanda L. ^[3] ; Damasio, Andre ^[3] ; Franco, Telma T. ^[1] ; Rabelo, Sarita C. ^[4]

Affiliation

^[1] Univ Estadual Campinas, Interdisciplinary Ctr Energy Planning, Cora Coralina 330, Campinas, SP - Brazil

^[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Giuseppe Maximo Scolfaro 10-000, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, UNICAMP, Campinas, SP - Brazil

^[4] Sao Paulo State Univ, Coll Agr Sci, Dept Bioproc & Biotechnol, UNESP, Ave Univ, 3780 Altos do Paraiso, Sao Paulo - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 313, OCT 2020.
• Web of Science Citations: 0

Abstract

Sugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers. (AU)

• FAPESP's process: 17/15477-8 - Development of a low severity pretreatment process for the production of sugarcane straw oligosaccharides
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Grantee: Telma Teixeira Franco
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/22669-0 - N-glycosylation and enzymes secretion in filamentous fungi
• Grantee: André Ricardo de Lima Damasio
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants