Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9

Author(s)

Ibanez-Redin, Gisela ^[1] ; Materon, Elsa M. ^{[2, 1]} ; Furuta, Roberto H. M. ^[1] ; Wilson, Deivy ^[1] ; do Nascimento, Gustavo Freitas ^[1] ; Melendez, Matias E. ^{[3, 4]} ; Carvalho, Andre L. ^[3] ; Reis, Rui Manuel ^{[3, 5, 6]} ; Oliveira Jr, Osvaldo N. ; Goncalves, Debora ^[7]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil

^[4] Pele Little Prince Res Inst, Little Prince Complex, BR-80250060 Curitiba, Parana - Brazil

^[5] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

^[6] Univ Minho, Med Sch, Life & eHlth Sci Res Inst ICVS, Braga - Portugal

^[7] Oliveira Jr, Jr., Osvaldo N., Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Microchimica Acta; v. 187, n. 7 JUL 1 2020.
• Web of Science Citations: 0

Abstract

Electrochemical immunosensors have been developed to determine the carbohydrate antigen 19-9 (CA19-9). They are based on screen-printed carbon electrodes (SPCEs) coated with layer-by-layer (LbL) films of carbon black (CB) and polyelectrolytes. Owing to a suitable choice of LbL film architecture, the procedures for immobilization of anti-CA19-9 antibodies on the electrode surfaces were straightforward. Mechanically flexible immunosensors were capable of detecting CA19-9 within a dynamic range of 0.01 to 40 U mL(-1) and a limit of detection of 0.07 U mL(-1) using differential pulse voltammetry. In addition to detecting CA19-9 at clinically relevant concentrations for pancreatic cancer in standard solutions, the immunosensors provide the determination of CA19-9 on cell lysate and human serum samples. Using LbL films led to immunosensors with superior performance compared to similar systems obtained by drop casting. The fabrication of this relatively simple, inexpensive platform is a demonstration that SPCEs modified with cost-effective materials are able to detect cancer biomarkers and may be adapted to other disposable immunosensors. (AU)

• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants