NAD(+)precursor increases aerobic performance in mice

Author(s):Show less -

Crisol, Barbara M. ^[1] ; Veiga, Camilla B. ^[2] ; Braga, Renata R. ^[1] ; Lenhare, Luciene ^{[1, 3]} ; Baptista, Igor L. ^[4] ; Gaspar, Rafael C. ^[1] ; Munoz, Vitor R. ^[1] ; Cordeiro, Andre V. ^[1] ; da Silva, Adelino S. R. ^{[5, 6, 7]} ; Cintra, Dennys E. ^[2] ; Moura, Leandro P. ^{[8, 1]} ; Pauli, Jose R. ^{[8, 1]} ; Ropelle, Eduardo R. ^{[8, 1, 3]}

Affiliation

^[1] Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Pedro Zaccaria, Pedro Zaccaria 1300, BR-13484350 Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP - Brazil

^[3] Univ Campinas UNICAMP, Dept Internal Med, Fac Med Sci, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Sch Appl Sci, Lab Cell & Tissue Biol, Limeira, SP - Brazil

^[5] Univ Sao Paulo, Med Sch, Ribeirao Preto, SP - Brazil

^[6] Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[7] Univ Sao Paulo, Postgrad Program Phys Educ & Sport, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[8] Univ Campinas UNICAMP, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Limeira, SP - Brazil

• Document type: Journal article
• Source: EUROPEAN JOURNAL OF NUTRITION; v. 59, n. 6, p. 2427-2437, SEP 2020.
• Web of Science Citations: 1

Abstract

Purpose Nicotinamide riboside (NR) acts as a potent NAD(+)precursor and improves mitochondrial oxidative capacity and mitochondrial biogenesis in several organisms. However, the effects of NR supplementation on aerobic performance remain unclear. Here, we evaluated the effects of NR supplementation on the muscle metabolism and aerobic capacity of sedentary and trained mice. Methods Male C57BL/6 J mice were supplemented with NR (400 mg/Kg/day) over 5 and 10 weeks. The training protocol consisted of 5 weeks of treadmill aerobic exercise, for 60 min a day, 5 days a week. Bioinformatic and physiological assays were combined with biochemical and molecular assays to evaluate the experimental groups. Results NR supplementation by itself did not change the aerobic performance, even though 5 weeks of NR supplementation increased NAD(+)levels in the skeletal muscle. However, combining NR supplementation and aerobic training increased the aerobic performance compared to the trained group. This was accompanied by an increased protein content of NMNAT3, the rate-limiting enzyme for NAD + biosynthesis and mitochondrial proteins, including MTCO1 and ATP5a. Interestingly, the transcriptomic analysis using a large panel of isogenic strains of BXD mice confirmed that theNmnat3gene in the skeletal muscle is correlated with several mitochondrial markers and with different phenotypes related to physical exercise. Finally, NR supplementation during aerobic training markedly increased the amount of type I fibers in the skeletal muscle. Conclusion Taken together, our results indicate that NR may be an interesting strategy to improve mitochondrial metabolism and aerobic capacity. (AU)

• FAPESP's process: 16/01089-3 - Evaluation of mtUPR and mito-nuclear imbalance in response to physical training: A multiorganic approach.
• Grantee: Eduardo Rochete Ropelle
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/07634-9 - Evaluation of mitonuclear imbalance and UPRmt in the skeletal muscle of exercise mice: The role JNK and PKR.
• Grantee: Eduardo Rochete Ropelle
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/05499-1 - Effects of nicotinamide Ribosoide supplementation on mitochondrial UPR in skeletal muscle and the aerobic performance of mice
• Grantee: Barbara Moreira Crisol
• Support Opportunities: Scholarships in Brazil - Master