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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Purification of rabies virus glycoprotein produced inDrosophila melanogasterS2cells: An efficient immunoaffinity method

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Author(s):
Pilatti, Livia [1, 2] ; Mancini Astray, Renato [1] ; Rocca, Mayra Pereira [3] ; Barbosa, Flavia Ferreira [1] ; Jorge, Soraia Attie Calil [1] ; Butler, Michael [4] ; de Fatima Pires Augusto, Elisabeth [2]
Total Authors: 7
Affiliation:
[1] Butantan Inst, Viral Immunol Lab, Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Sci & Technol Inst, Sao Jose Dos Campos, SP - Brazil
[3] Sao Paulo State Univ UNESP, Med Sch, Botucatu, SP - Brazil
[4] Natl Inst Biotechnol Res & Training NIBRT, Dublin - Ireland
Total Affiliations: 4
Document type: Journal article
Source: BIOTECHNOLOGY PROGRESS; v. 36, n. 6 AUG 2020.
Web of Science Citations: 0
Abstract

Most rabies vaccines are based on inactivated virus, which production process demands a high level of biosafety structures. In the past decades, recombinant rabies virus glycoprotein (RVGP) produced in several expression systems has been extensively studied to be used as an alternative vaccine. The immunogenic characteristics of this protein depend on its correct conformation, which is present only after the correct post-translational modifications, typically performed by animal cells. The main challenge of using this protein as a vaccine candidate is to keep its trimeric conformation after the purification process. We describe here a new immunoaffinity chromatography method using a monoclonal antibody for RVGP Site II for purification of recombinant rabies virus glycoprotein expressed on the membrane ofDrosophila melanogasterS2 cells. RVGP recovery achieved at least 93%, and characterization analysis showed that the main antigenic proprieties were preserved after purification. (AU)

FAPESP's process: 18/10538-1 - Characterization of recombinant antigens and immune response of a new rabies vaccine candidate
Grantee:Renato Mancini Astray
Support type: Regular Research Grants
FAPESP's process: 12/24647-0 - Purification and characterization of rabies virus glycoprotein expressed by S2 (Drosophila melanogaster) and recombinant Semliki Forest Virus systems
Grantee:Renato Mancini Astray
Support type: Regular Research Grants