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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of dentin biomodification delivered by experimental acidic and neutral primers on resin adhesion

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Author(s):
Trevelin, Livia Tosi [1, 2] ; Alania, Yvette [1] ; Mathew, Mathew [1] ; Phansalkar, Rasika [3, 4] ; Chen, Shao-Nong [3, 4] ; Pauli, Guido F. [3, 4] ; Bedran-Russo, Ana K. [1]
Total Authors: 7
Affiliation:
[1] Univ Illinois, Coll Dent, Dept Restorat Dent, Chicago, IL - USA
[2] Univ Sao Caetano Do Sul, Coll Dent, Dept Operat Dent, USCS, Sao Caetano do Sul - Brazil
[3] Univ Illinois, Coll Pharm, Program Collaborat Res Pharmaceut Sci, Chicago, IL 60612 - USA
[4] Univ Illinois, Coll Pharm, Dept Pharmaceut Sci, Chicago, IL - USA
Total Affiliations: 4
Document type: Journal article
Source: Journal of Dentistry; v. 99, AUG 2020.
Web of Science Citations: 0
Abstract

Objectives: Proanthocyanidins (PACs) are biocompounds mimicking native collagen cross-links. The effective and practical delivery of any biocompound is pivotal for clinical usage. The aim was to investigate the dentin biomodification and effective formation of dentin-resin biointerfaces of two highly bioactive PAC-rich extracts, Vitis vinifera (Vv) and Camellia sinensis (Cs), delivered using neutral (NP) or acidic (AP) rinse-out primer approaches. Methods: The depth of dentin demineralization (optical profilometry), dentin biomodification (apparent modulus of elasticity, collagen auto-fluorescence) and properties of dentin-resin interfaces (microtensile bond strength - mu TBS, and micro-permeability) were investigated. NP consisted of either 15% Vv or Cs applied for 60 s after surface etching; while AP contained 15% Vv or Cs in either 35% glycolic acid or tartaric acid applied for 30 s or 60 s. Data were analyzed using ANOVA and post-hoc tests (alpha = 0.05). Results: The depth of demineralization was statistically higher when applied for 60 s, regardless of rinse-out primer approach (p < 0.001). Compared to the AP strategy, NP exhibited statistically higher apparent modulus of elasticity, regardless of PAC extract (p < 0.001). Highest mu TBS were obtained for NPVv, which were statistically similar to AP(GAVv), when applied for 60 s (p < 0.001); both resulted in a dramatic decrease of the interfacial permeability. NPCs, group showed the lowest mu TBS (p < 0.001). Conclusions: A combination of high bond strength and low micro-permeability can be accomplished using glycolic acid with the mid- and high-PAC oligomer enriched extract (Vv). Cs extract containing mostly catechins and dimeric PACs, was found unsuitable for resin-dentin adhesion despite exhibiting high initial dentin biomodification. Clinical significance: This study provides a new conceptual delivery of PAC-mediated dentin biomodification and conservative dentin surface etching using rinse-out primers. The strategy requires a specific combination of PAC source, alpha-hydroxy acid, and application time. (AU)

FAPESP's process: 15/12651-1 - Influence of temporal width pulse of Er:YAG laser in dentin morphology, hybrid layer and bond strength
Grantee:Lívia Tosi Trevelin
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/18118-6 - News strategies to prevent adhesive interface degradation
Grantee:Lívia Tosi Trevelin
Support type: Scholarships abroad - Research Internship - Doctorate